Plant Virology Protocols系列图书下载
植物病毒学实验书籍3本PDF下载Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses
Springer Science+Business Media New York 2015
Uyeda, Ichiro, Masuta, Chikara (Eds.)
3rd ed. 2015, XI, 292 p. 70 illus., 32 illus. in color.声明:文件来自网络,仅供论坛内部学习交流之用,请注意版权保护,勿外传。下载地址:**** Hidden Message *****
A product of Humana Press
[*]Includes new cutting-edge methods and protocols in plant virology
[*]Provides step-by-step detail essential for reproducible results
[*]Contains key notes and implementation advice from the experts
Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the utilization of meta-genome sequencing and global gene expression analyses for the search and identification of viruses, as well as the elucidation of host responses to viral infection, construction methods of infectious cDNAs, and methods relevant to plant virus control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses will be an invaluable guide to researchers working in the field of plant sciences.
Plant Virology Protocols: From Viral Sequence to Protein Function
[*]出版社: Humana Press Inc.; 2nd ed. Softcover of orig. ed. 2008 (2010年10月28日)
[*]平装: 677页
[*]语种: 英语
[*]ISBN: 1617377945
[*]ASIN: 1617377945
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[*]下载地址:http://dl.vmall.com/c0g3a1rmv0
[*]密码:BBS2
[*]Following the considerable success of the first edition of "Plant Virology Protocols", this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section of "Plant Virology Protocols, Second Edition" presents the most novel methods and protocols to allow researchers more effective approaches to study plant viruses. The many subjects covered in this important new edition range from techniques for investigating viral nucleic acid sequence, protein analysis and investigation of protein function, techniques for microscopy/GFP visualization and analysis/protein tagging/generation of infectious clones and other such tools, as well as areas of genomics and interactions with host factors and plant-based studies. "Plant Virology Protocols, Second Edition" - from viral sequence to protein function is indispensable to laboratory investigators studying plant viruses.
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Plant Virology Protocols: From Virus Isolation to Transgenic Resistance
[*]出版社: Humana Press Inc.; 1998 (1998年2月24日)
[*]精装: 571页
[*]语种: 英语
[*]ISBN: 0896033856
[*]ASIN: 0896033856
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[*]下载地址 http://dl.vmall.com/c0hupgjnui
[*]密码:BBS1
[*]The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.
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目录 TABLE OF CONTENTS
1. Detection of Plant Viruses in Mixed Infection by a Macroarray-Assisted Method
2. RT-PCR and Real-Time RT-PCR Methods for the Detection of Potato Virus Y in Potato Leaves and Tubers
3. New Method to Isolate Total dsRNA
4. Multiplex RT-PCR Method for the Simultaneous Detection of Nine Grapevine Viruses
5. Detection Methods for Rice Viruses by a Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)
6. Real-Time PCR Protocols for the Quantification of the Begomovirus Tomato Yellow Leaf Curl Sardinia Virus in Tomato Plants and in Its Insect Vector
7. Detection and Analysis of Non-Retroviral RNA Virus-Like Elements in Plant, Fungal, and Insect Genomes
8. Detection of Plant Viruses in Natural Environments by Using RNA-Seq
9. Cloning and Profiling of Small RNAs from Cucumber Mosaic Virus Satellite RNA
10. Drawing siRNAs of Viral Origin out from Plant siRNAs Libraries
11. Viral Detection by High-Throughput Sequencing
12. Analysis and Application of Viroid-Specific Small RNAs Generated by Viroid-Inducing RNA Silencing
13. Detection and Characterization of Mycoviruses in Arbuscular Mycorrhizal Fungi by Deep-Sequencing
14. SuperSAGE as an Analytical Tool for Host and Viral Gene Expression
15. Microarray Analysis of R-Gene-Mediated Resistance to Viruses
16. Construction of Infectious cDNA Clones Derived from the Potyviruses Clover Yellow Vein Virus and Bean Yellow Mosaic Virus
17. Virus-Induced Gene Silencing of N Gene in Tobacco by Apple Latent Spherical Virus Vectors
18. Simplified Methods for the Construction of RNA and DNA Virus Infectious Clones
19. Efficient Double-Stranded RNA Production Methods for Utilization in Plant Virus Control
20. Detection of Plant Virus in Meristem by Immunohistochemistry and In Situ Hybridization
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