中科院武汉病毒所陈新文课题组关于杆状病毒研究新进展
本帖最后由 virologica 于 2016-11-8 16:53 编辑苜蓿银纹夜蛾核型多角体病毒（AcMNPV）在感染昆虫宿主细胞过程中，会将大量病毒蛋白质与宿主蛋白质转移到胞核，用于在胞核内完成病毒基因组复制，基因转录与新生核壳体装配等病毒复制过程。然而对于AcMNPV如何诱导大量蛋白质发生核聚集的机制未知。
CRM1是真核细胞内最重要的蛋白质出核转运通路。通过构建AcMNPV瞬时表达文库，武汉病毒所科研人员筛选到AcMNPV晚期基因产物Ac34可以特异性关闭宿主细胞依赖CRM1的蛋白质出核转运通路，从而在病毒感染晚期，将与病毒复制相关的蛋白质，包括肌动蛋白聚合因子Arp2/3复合物等富集在胞核内，用于核壳体装配等重要过程。相比过去发现的多种病毒破坏宿主细胞的蛋白质入核转运通路（主要是抑制干扰素的产生），该研究首次发现杆状病毒可以特异性破坏宿主细胞的出核转运通路，从而为病毒复制服务。目前科研人员正在深入探索Ac34关闭CRM1的分子机制，以期测底解析AcMNPV诱导大量蛋白质发生核聚集的原因。
该研究成果发表于PLOS Pathogens。第一作者为穆敬芳博士，通讯作者为陈新文研究员、王云副研究员。
AbstractActin, nucleation-promoting factors (NPFs), and the actin-related protein 2/3 complex (Arp2/3) are key elements of the cellular actin polymerization machinery. With nuclear actin polymerization implicated in ever-expanding biological processes and the discovery of the nuclear import mechanisms of actin and NPFs, determining Arp2/3 nucleo-cytoplasmic shuttling mechanism is important for understanding the function of nuclear actin. A unique feature of alphabaculovirus infection of insect cells is the robust nuclear accumulation of Arp2/3, which induces actin polymerization in the nucleus to assist in virus replication. We found that Ac34, a viral late gene product encoded by the alphabaculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), is involved in Arp2/3 nuclear accumulation during virus infection. Further assays revealed that the subcellular distribution of Arp2/3 under steady-state conditions is controlled by chromosomal maintenance 1 (CRM1)-dependent nuclear export. Upon AcMNPV infection, Ac34 inhibits CRM1 pathway and leads to Arp2/3 retention in the nucleus.
原文链接：http://dx.doi.org/10.1371/journal.ppat.1005994
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