Chromatography lecture(Introduction)
Chromatography lecture and my learning diariesThe chromatography is a collective terms for separation of mixtures techniques. The sample (Mixtures) is dissolved in the mobile phase, which carried it through the stationary phase. The separation was achieved by the difference of travel time/speeds. Several chromatography methods have been developed during the past time including paper chromatography, gas chromatography, and high performance liquid chromatography (HPLC). In this lecture, the teacher mainly introduced the principle of HPLC.
HPLC is a technique to separate a mixture of compounds in analytical chemistry and biochemistry with the purpose of identifying, quantifying and purifying the individual component. The instruments consists of PUMP (moves the mobile phase and sample through the column), injector (add samples), column and detector.
Different liquid chromatography including gel filtration, ion exchange, affinity and reversed phase chromatography are utilized according to the protein/peptide properties including size, net surface charge, hydrophobicity, respectively. Different methods can be combined according to the purpose, but the most important is keep it simple because quite a lot of samples are lost during the process.
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