标题: Virologica Sinica 精彩论文抢先看！ [打印本页] 作者: lishaojie 时间: 2015-12-7 17:01 标题: Virologica Sinica 精彩论文抢先看！ Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF). P2 L. a! s5 y+ p, V( | R Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi* & D! @1 k. X* l9 Q0 T# O% L4 q3 H% A1 ~; Y" R Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.7 g5 K' X) V6 Z* o3 O) ]
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little * W0 [. F9 ?5 i& d b( e B. linformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a 6 e; ^7 m! D' Y' Ekey role in controlling viral infections. In this study, we report the cloning, expression, and % ]6 C4 e* ~" e2 l6 Ibiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We + O& o& T+ D( L5 X. v" `$ L2 Hdemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from9 u7 }8 @% m3 P9 _$ v
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant* a, @+ k) J& \, d" z) \) b6 [
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat " N9 C- Q# b2 Y# vspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral % ]: K- a- B/ Z0 G5 Wactivity in microbats, which has important implications for virus interactions with these hosts. 3 _: @# d. a5 J3 \9 c* @' y% v: ?5 J4 T) @3 X' u0 h Keywords: bat; interferon; IFN-stimulated genes; antiviral activity/ k- Q7 s* r3 Z1 B4 j
]/ A' b" D R! t, | 4 Q* A& S7 q7 G$ B5 I5 [; r$ S中文信息：0 N* v, F A! R. u/ E% a
$ E& v7 A& n4 Q: t5 C大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测: }+ c- c7 w3 G: r 9 p" h, I4 i, V, k9 K7 M" C1 P% s: ~+ O7 Z( N) N
梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽* 2 E/ l2 R2 j' R! q2 C7 [8 V$ R% l( B9 i- N" e9 ^" U
# S) s$ ~* i( A5 Y, ~$ J, V 摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。 C, f4 S U* q- ?& a& [
m( }" x" b7 G5 N8 \+ z1 \# @1 [! `* B( t3 m* K4 X 关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR r0 Q" y7 X6 w: Q& n