培养基更换 每2-3天一次
冻存液 95%培养基；5%,DMSO；culture medium 95%; DMSO, 5%；
培养条件 ATCC培养基：90%的MEM Eagle，加入2mM L-谷氨酰胺，1.5g/L碳酸氢钠，0.1 mM必需氨基酸，1.0 mM 丙酮酸钠；10%加热灭活的马血清
37℃培养作者: wwwkkk83 时间: 2016-8-30 11:20
The 293FT Cell Line is derived from the 293F Cell Line and stably
expresses the SV40 large T antigen from the pCMVSPORT6TAg.neo plasmid.
Expression of the SV40 large T antigen is controlled by the human cytomegalovirus
(CMV) promoter and is high-level and constitutive. Studies have demonstrated maximal virus production in human 293 cells expressing SV40 large T antigen (Naldini et al., 1996), making the 293FT Cell Line a particularly suitable host for generating lentiviral constructs using the ViraPower Lentiviral Expression System available from Invitrogen.
The 293 Cell Line is a permanent line established from primary embryonal human
kidney transformed with sheared human adenovirus type 5 DNA (Graham et al.,
1977; Harrison et al., 1977). The E1A adenovirus gene is expressed in these cells
and participates in transactivation of some viral promoters, allowing these cells to
produce very high levels of protein.
The 293-F Cell Line available from Invitrogen (Catalog no. 11625) is a fastgrowing
variant of the 293 cell line, and was originally obtained from Robert Horlick at Pharmacopeia.作者: wwwkkk83 时间: 2016-8-30 11:23
如果要使用 ViraPower™ 慢病毒表达系统产生高滴度的慢病毒，293FT 细胞系是理想的选择。293FT 细胞系是生长快速，非常易于转染的克隆分离物，来自通过 SV40 大 T 抗原转化的人胚胎肾细胞。 ViraPower™ 表达载体和 ViraPower™ 慢病毒包装混合物 (ViraPower™ Lentiviral Packaging Mix) 共转染进 293FT 细胞中时，可形成高水平的病毒 RNA 及包装所需的 gag/pol 和 rev 蛋白。
