【书名版次】: Adeno-Associated Virus: Methods and Protocols (Methods in Molecular Biology)
【作 者】:Richard O. Snyder (Editor), Philippe Moullier (Editor)7 H1 p+ S6 w: O1 s
【出 版 社】: Humana Press; 2011 edition D S0 y- M \6 E
【出版日期】: (November 30, 2011)
【简 介】: Today, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding — or at least an appreciation of — the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Protocols, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Adeno-Associated Virus: Methods and Protocols provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.
【目 录】:
Hardcover: 475 pages3 M( |# y) M- D" c
Language: English: [4 b% D# {; t
ISBN-10: 16177936984 p5 x. _, B9 {- ^# @5 N
ISBN-13: 978-1617793691# S( T0 K. s+ V! d! z+ Z( H
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