2.RNAi目标序列的选取原则：
（1）siRNAs with lower G/C content (35-55%) are more active than those with G/C content higher than 55%；
（2）Beginning with the AUG start codon of your transcript, scan downstream for AA dinucleotide sequences. Record the occurrence of each AA and the 3' adjacent 19 nucleotides as potential siRNA target sites. Tuschl, et al. recommend against designing siRNA to the 5' and 3' untranslated regions (UTRs) and regions near the start codon (within 75 bases) as these may be richer in regulatory protein binding sites. UTR-binding proteins and/or translation initiation complexes may interfere with binding of the siRNP endonuclease complex.
（3）Compare the potential target sites to the appropriate genome database (human, mouse, rat, etc.) and eliminate from consideration any target sequences with significant homology to other coding sequences.
3.siRNA的合成：
(1)IN VITRO: siRNAs generated in vitro by transcription with T7 DNA polymerase;
(2)IN VIVO: Short hairpin RNAs that are transcribed in vivo from vectors containing the human U6 promoter.
功能基因组资源：蛋白质组
包括常用资源，蛋白-蛋白相互作用数据库，蛋白质家族数据库，分析软件等 http://www.biosino.org/bioinformatics/010929-6.htm
Metabolic Pathways
14 Cell Cycle 6 Signal Transduction 12 ExPASy Biochemical Pathways Digitized version of wall charts courtesy Boehringer Mannheim et al,divided into Metabolic Pathways and Cellular and Molecular Processes,maintained by the Swiss Institute of Bioinformatics,Geneva,Switzerland.Diagram of steps in Krebs tricarb http://www.maninnet.com/phod.php ... Metabolic_Pathways/
Chemical Network -- Chemical Industry Directory
All Categories Just in this category Biochemicals About 78records found Showing 41 60of 78 3 A company operating in the forefront of genetic engineering as well as DNA,RNA and protein research.This is the ExPASy Expert Protein Analysis System proteomics server of t http://www.chemnet.com/dir/Europe/Pharmaceuticals,_biochemicals_and_intermediates/Biochemicals/index3.html
The guide:
Step 1 obtaining a sequence of interest If you have a sequence of interest proceed to step 2.Once you obtained your sequence of interest YSI save it in a file using the Save As command of your browser.Search a public version of full Medline for topics of interest US Search for sequences of interest US Search protein and nucleic acid databas http://www.biosino.org/mirror/bi ... .il/gdp/gdlist.html
Bioinformatics and XML
XML因其将数据信息本身的存储与关联与表现形式相分离，强大的可扩展性，本身层次清晰的树形结构特性以及跨平台 跨语言的特性而成为良好网际语言，并在各种数据和存取工作中大显神通。生物信息学的发展同样引入了XML技术。下面这篇文章是一个专业性的评论，文章分析了现在XML技术在生物信息学 .. http://www.biosino.org/bioinformatics/011212-2.htm