介绍了植物病毒高通量测序方法及应用，多种测未知病毒的方法及其缺点。看上去比较通俗易懂
Plant Virus Metagenomics: Biodiversity and Ecology
Marilyn J. Roossinck
Abstract
Viral metagenomics is the study of viruses in environmental samples, using next generation sequencing that produces very large data sets. For plant viruses, these studies are still relatively new, but are already indicating that our current knowledge grossly underestimates the diversity of these viruses. Some plant virus studies are using thousands of individual plants so that each sequence can be traced back to its precise host. These studies should allow for deeper ecological and evolutionary analyses. The finding of so many new plant viruses that do not cause any obvious symptoms in wild plant hosts certainly changes our perception of viruses and how they interact with their hosts. The major difficulty in these (as in all) metagenomic studies continues to be the need for better bioinformatics tools to decipher the large data sets. The implications of this new information on plant viruses for international agriculture remain to be addressed.
这是作者给出的要点：
SUMMARY POINTS
1. Plant virus metagenomics is a rapidly developing field that is changing the way we
understand plant viruses.
2. Ecogenomic studies, in which individual plants are sampled followed by deep-sequence
analysis, will provide data for in-depth plant virus ecology studies.
3. Plant viruses have been found in metagenomic studies from a number of nonplant environments,
including mammalian guts, wastewater, and fresh water.
4. Bioinformatics has not kept up with the generation of data in virus metagenomics.
5. The discovery of numerous novel viruses in plants has implications for food biosecurity
and international agriculture.