There is poly A tail! But nobody will put the a tail to the databank, I think this is because nobody knows how many A are there in the virus genome.
I have used oligo d T to amplify several potyviridae virus, it works fine without any problem.
The reason why you haven't get the product maybe you have the bad cDNA although you could amplify with the specific primers. I have the experience that if I do the RT using RANDOM primer, it is difficult to get product using oligo d T.作者: donggua 时间: 2015-7-19 23:50
牵着乌龟追兔子回复：
有些单链正义RNA病毒3端有PolyA尾，有这种尾的5端一般都是VPg