Introduction Western blotting, also known as immunoblotting, is a well established and widely used technique for the detection and analysis of proteins. The method is based on building an antibody:protein complex via specific binding of antibodies to proteins immobilized on a membrane and detecting the bound antibody with one of several detection methods. The Western blotting method was first described in 1979 (1) and has since become one of the most commonly used methods in life science research. The aim of this handbook is to guide and inspire beginners as well as experts toward successful Western blotting. It provides expertise and support, taking you through the complete Western blotting workflow, from sample preparation to detection and analysis. Chapters 2 to 8 follow the Western blotting workflow step by step and describe theoretical as well as practical aspects of the technique together with useful hints and tips. Examples of typical applications as well as some new approaches to Western blotting are described in Chapter 9. Moreover, protocols and recipes are provided in Chapter 11, including a recommended standard Western blotting procedure, to help you design and run your experiments. Over the past decade, improved detection methods and software have brought quantitative analysis to Western blotting and this handbook provides examples and protocols to help you adapt your methods and obtain more quantitative data from your Western blots. Finally, a troubleshooting guide is provided in Chapter 10, just in case! A series of methodology handbooks is available from GE Healthcare, many of which cover topics closely connected to Western blotting, such as sample preparation or 2-D gel electrophoresis. Where appropiate, we refer to these handbooks in the text and provide links to the PDF versions in the on-line version of this handbook. 这是GE公司的资料，上课老师推荐的也是这资料，尤其对初学者。理论加实践更容易理解应用。 https://skydrive.live.com/redir.aspx?cid=1bf2fb7679cabbd5&resid=1BF2FB7679CABBD5!157&parid=1BF2FB7679CABBD5!155&authkey=!AHZr-ktUYBV9p6M |
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