(1)世界卫生组织 (World Health Organization)。"Cumulative Number of Confirmed Human Cases of Avian Influenza A/(H5N1) Reported to WHO." 于2008年8月21日存档。作者: cao1976 时间: 2015-9-10 14:04
rojjer发表于 2008-8-28 09:19:
送上Novavax的相关研究论文：
Cross-Clade Protective Immune Responses to Influenza Viruses with H5N1 HA and NA Elicited by an Influenza
Virus-Like Particle
Rick A. Bright1*, Donald M. Carter2, Corey J. Crevar2, Franklin R. Toapanta2, Jonathan D. Steckbeck2, Kelly S. Cole2, Niranjan M. Kumar1, Peter
Pushko1, Gale Smith1, Terrence M. Tumpey3, Ted M. Ross2*
1 Novavax, Inc., Rockville, Maryland, United States of America, 2 Center for Vaccine Research, University of Pittsburgh, Pittsburgh, Pennsylvania,
United States of America, 3 Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America
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Background. Vaccination is a cost-effective counter-measure to the threat of seasonal or pandemic outbreaks of influenza. To address the need for improved influenza vaccines and alternatives to egg-based manufacturing, we have engineered an influenza virus-like particle (VLP) as a new generation of non-egg or non-mammalian cell culture-based candidate vaccine.Methodology/Principal Findings. We generated from a baculovirus expression system using insect cells, a non-infectious recombinant VLP vaccine from both influenza A H5N1 clade 1 and clade 2 isolates with pandemic potential. VLPs were administered to mice in either a one-dose or two-dose regimen and the immune responses were compared to those induced by recombinant hemagglutinin (rHA). Both humoral and cellular responses were analyzed. Mice vaccinated with VLPs were protected against challenge with lethal reassortant viruses expressing the H5N1 HA and NA, regardless if the H5N1 clade was homologous or heterologous to the vaccine. However, rHA-vaccinated mice showed considerable weight loss and death following challenge with the heterovariant clade virus. Protection against death induced by VLPs was independent of the prechallenge HAI titer or cell-mediated responses to HA or M1 since vaccinated mice, with low to undetectable cross-clade HAI antibodies or cellular responses to influenza antigens, were still protected from a lethal viral challenge. However, an apparent association rate of antibody binding to HA correlated with protection and was enhanced using VLPs, particularly when delivered intranasally, compared to rHA vaccines. Conclusion/Significance. This is the first report describing the use of an H5N1 VLP vaccine created from a clade 2 isolate. The results show that a non-replicating virus-like particle is effective at eliciting a broadened, cross-clade protective immune response to proteins from emerging H5N1 influenza isolates giving rise to a potential pandemic influenza vaccine candidate for humans that can be stockpiled for use in the event of an outbreak of H5N1 influenza