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这次疫苗候选毒株还是由传统方法制备出来的，由New York Medical College的病毒学家Doris Bucher制备。与季节性流感不同的是，她不是直接使用PR8，而是用了所谓的NYMC X-157（NYMC as in "New York Medical Center;" it's a hybrid of an H3N2 seasonal virus and the so-called "Puerto Rico strain," A/PR/8/34, that's used to speed the growth of seasonal flu vaccine），当然只是为了避免新h1n1与PR8可能产生的交叉反应。她们获得了四株病毒。
同时韩国学者Sang-heui也利用的反向遗传技术获得了一个病毒株，不过CDC却没有同意应用（原因是：the methods Sang-heui used were not suitable and that his virus was not considered a viable candidate.）作者: cao1976 时间: 2015-9-11 08:58
gywmail发表于 2009-5-31 18:58：
这次疫苗候选毒株还是又传统方法制备出来的，由New York Medical College的病毒学家Doris Bucher制备。与季节性流感不同的是，她不是直接使用PR8，而是用了所谓的NYMC X-157（NYMC as in "New York Medical Center;" it's a hybrid of an H3N2 seasonal virus and the so-called "Puerto Rico strain," A/PR/8/34, that's used to speed the growth of seasonal flu vaccine），当然只是为了避免新h1n1与PR8可能产生的交叉反应。她们获得了四株病毒。
解释：
For the H1N1, we don't want to use the Puerto Rico strain, as it itself is also an H1N1 virus, so you risk running into some cross-reactivity. Having a donor strain from a different flu subtype, which is immunologically more different, just makes the entire antibody-selection process much easier. Four or five years ago, we realized we needed a good donor strain for H1N1 targets, so we used a strain that we called, in honour of our institution, NYMC X-157, a reassortant between the PR/8/34 and an H3N2 strain. X-157 was the H3N2 component in the 2005-06 vaccine. It has six genes from the high-yield Puerto Rico strain, so although it's essentially a high-yield PR/8/34, its surface is largely H3N2. So to the immune system it looks like an H3N2 strain, making it a better donor strain when you are dealing with an H1N1 target virus. And X-157 has shown great ability to reassort with H1N1 targets.作者: cao1976 时间: 2015-9-11 08:58
Rojjer发表于 2009-6-2 10:17：
Candidate vaccine viruses are being developed by a number of laboratories as indicated by WHO .
A/California/7/2009 (egg isolate) – classical reassortment and reverse genetics