通过与中国疾病预防控制中心高福院士、梁米芳研究员以及军事医学科学院曹务春研究员合作,阎锡蕴课题组成功将该技术应用于埃博拉、新布尼亚、流感等病毒的检测。目前这项这项成果已经发表在生物检测领域的专业期刊《Biosensors and Bioelectronics》上,并立即被评为ScienceDirect热点文章(Top 25 Hottest Articles);ELSEVIER同时配发了专题评论,称该技术克服了传统试纸条法因灵敏度低而长期应用受限的瓶颈问题,有望成为控制埃博拉传播的利器。
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英文摘要:
Biosensors and Bioelectronics doi:10.1016/j.bios.2015.05.025
Nanozyme-strip for rapid local diagnosis of Ebola
Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, by using Fe3O4 magnetic nanoparticle (MNP) as a nanozyme probe, we developed a MNP-based immunochromatographic strip (Nanozyme-strip), which detects the glycoprotein of Ebola virus (EBOV) as low as 1 ng/mL, which is 100-fold more sensitive than the standard strip method. The sensitivity of the Nanozyme-strip for EBOV detection and diagnostic accuracy for New Bunyavirus clinical samples is comparable with ELISA, but is much faster (within 30 min) and simpler (without need of specialist facilities). The results demonstrate that the Nanozyme-strip test can rapidly and sensitively detect EBOV, providing a valuable simple screening tool for diagnosis of infection in Ebola-stricken areas.