参考文献
1 Yu AS，Keeffe EB.Management of hepatocellular carcinoma.Rev Gasˉtroenterol Disord，2003，3（1）:8-24.
2 Pisani P，Parkin DM，Bray F，et al.Estimates of worldwide mortality from25cancers in1990.Int J Caner，1999，83:18-29.
3 陈瑞铭.一株人体肝癌细胞株的建立和一些初步的观察.肿瘤研究论文集，1962，39-47.
4 Alexander JJ，Bey EM，Geddes EW，et al.Establishment of a continuˉously growing cell line from primary carcinoma of the liver.S Afr Med J，1976，50（54）:2124-2128.
5 Morris KM，Aden DP，Knowles BB，Colten HR.Complement biosynˉthesis by the human hepatoma-derived cell line HepG2.J Clin Invest，1982，70（4）:906-913.
6 王金兵，朱德厚，叶秀珍，等.启东肝癌细胞系QGY-7703的建立及其特征.中华肿瘤杂志，1981，3（4）:243.
7 董荣春，周荣华，吕发度，等.SMMC-7721人肝癌细胞株的建立及其生物学特性的初步观察.第二军医大学学报，1980，（1）:5-9.
8 郭秀婵，蓝祥英，周玲，等.乙型肝炎病毒和黄曲霉素协同作用诱发人肝细胞癌细胞株的建立.病毒学报，2001，17（3）.205-209.
9 Aoki Y，Aizaki H，Shimoike T，et al.A human liver cell line exhibits efficient translation of HCV RNAs produced by a recombinant adenovirus expressing T7RNA polymerase.Virology，1998，250:140-150.
10 Tian J，Tang ZY，Ye SL，et al.Newhuman hepatocellular carcinoma（HCC）cell line with highly metastatic potential（MHCC97）and its expressions of the factors associated with metastasis.Br J Cancer，1999，81（5）:814-821.
11 Li Y，Tang ZY，Ye SL，et al.Establishment of cell clones with difˉferent metastatic potential from the metastatic hepatocellular carcinoma cell line MHCC97.World J Gastroenterol，2001，7（5）:630-636.
12 吴小鹏，王占民，刘博，等.外生型肝癌细胞系的建立及生物学特性研究.中华外科杂志，2002，40（8）:616-617.
13 Murakami T，Yano H，Maruiwa M，et al.Establishment and characˉterization of a human combined hepatocholangiocarcinoma cell line and its heterologous transplantation in nude mice.Hepatology，1987，7:551-556.
14 Sing GK，Pace R，Prior S，et al.Establishment of a cell line from a hepatocellu-lar carcinoma from a patientwith hemochromatosis.Hepˉatology，1994;20:74-81.
15 Dippold WG，Dienes HP，Knuth A，et al.Hepatocellular carcinoma after thorotrast exposure:establishment of a new cell line（Mz-Hep-1）.Hepatology，1985，5（6）:1112-1119.
16 Tsurimoto T，Fujiyama A，Matsubara K.Stable expression and repliˉcation of hepatitis B virus genome in an integrated state in a human hepatoma cell line trans-fected with the cloned viral DNA.Proc Natl Acad Sci U S A，1987，84（2）:444-448.
17 Song ZQ，Hao F，Min F，et al.Hepatitis C virus infection of human hepa-toma cell line7721in vitro.World J Gastroenterol，2001，7（5）:685-689.
18 杨俊霞，汤为学.人肝癌顺铂耐药细胞系的建立及其生物学特征.癌症，2002，21（8）:872-876.
19 张洪新，王执民，郭卫平，等.耐药人肝癌细胞模型—7721/Adm的建立.第四军医大学学报，2000，21（4）.425-429.
20 Ebinuma H，Saito H，Kosuga M，et al.Reduction of c-myc expresˉsion by an antisense approach under Cre/loxP switching induces apopˉtosis in human liver cancer cells.J Cell Physiol，2001，188（1）:56-66.
21 Li MS，Li PF，He SP，et al.The promoting molecular mechanism of alpha-fetoprotein on the growth of human hepatoma Bel7402cell line.World J Gastroenterol，2002，8（3）:469-475.
22 Obora A，Shiratori Y，Okuno M，et al.Synergistic induction of apopˉtosis by acyclic retinoid and interferon-beta in human hepatocellular carcinoma cells.Hepatology，2002，36（5）:1115-1124.
23 Wang Z，Ruan YB，Guan Y，et al.Expression of IGF-II in early experimental hepatocellular carcinomas and its significance in early diˉagnosis.World J Gastroenterol，2003，9（2）:267-270.
24 Favoulet P，Cercueil JP，Faure P，et al.Increased cytotoxicity and stability of Lipiodol-pirarubicin emulsion compared to classical doxˉorubicin-Lipiodol:potential advantage for chemoembolization of unreˉsectable hepatocellular carcinoma.Anticancer Drugs，2001，12（10）:801-806.
25 Abiru S，Nakao K，Ichikawa T，et al.Aspirin and NS-398inhibit hepatocyte growth factor-induced invasiveness of human hepatoma cells.Hepatology，2002，35（5）:1117-1124.
26 Bouchard MJ，Puro RJ，Wang L，et al.Activation and inhibition of cellular calcium and tyrosine kinase signaling pathways identify targets of the HBx protein involved in hepatitis B virus replication.J Virol，2003，77（14）:7713-7719.
27 Alisi A，Giambartolomei S，Cupelli F，et al.Physical and functional interaction between HCV core protein and the different p73isoforms.Oncogene，2003，22（17）:2573-2580.
28 Wood RP，Katz SM，Ozaki CF，et al.Extracorporeal liver assist deˉvice（ELAD）:a preliminary report.Transplant Proc，1993，25（4Supˉpl3）:53-54 作者: wwwkkk83 时间: 2019-1-9 08:38
PLC/PRF/5细胞系又称Alexander细胞系,是一株能稳定持续产生HBsAg的人肝癌细胞株。虽然国内外曾先后自肝癌组织建成不少细胞株,但能有乙型肝炎病毒抗原表达者仅有本株、Mahlavu株(由Pro-zesky 1973年建立)和Aden“建立的另一株,而其中以PLC/PRF/5(以下简称PLC) 产生HBsAg最稳定,量最多,研究最广。自从1976年Macnab报道以来,PLC已成为国际上研究乙型肝炎病毒,乙肝病毒与肝癌关系的一个重要实验模型。
PLC/PRF/5 (ATCC® CRL-8024™) https://www.atcc.org/Products/All/CRL-8024.aspx
Organism Homo sapiens, human
Tissue liver
Cell Type Alexander cells
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain hepatitis B]
Disease hepatoma
Genes Expressed hepatitis virus B surface antigen (HBsAg)
Cellular Products hepatitis virus B surface antigen (HBsAg)
Comments The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline.
The cells secrete HBsAg.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation culture medium 95%; DMSO, 5%
Culture Conditions Temperature: 37°C
Name of Depositor WJ McAleer
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References Monjardino J, Crawford E. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. PubMed:223321
Skelly J, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-618, 1979. PubMed: 233138
Knowles B. et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209(4455): 497-499, 1980. PubMed: 6248960
Alexander J, et al. Adaptation of cells derived from human malignant tumours to growth in vitro. S. Afr. J. Med. Sci. 41(2):89-98, 1976. PubMed:184551
Gilligan A, et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp. Cell Res. 200: 235-241, 1992. PubMed: 1315281
Alexander JJ, et al. Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. Med. J. 50: 2124-2128, 1976. PubMed: 63998
Organism Homo sapiens, human
Tissue liver / ascites
Cell Type Endothelial
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 52 years
Gender male
Ethnicity Caucasian
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype The cell line is aneuploid human (XX), with chromosome counts in the hypotriploid range. Normal chromosomes N1, N13, N14, N15, and N16 are clearly under-represented with respect to the copy number of other normal chromosomes, while chromosome N7, N12, and N17 tend to be over-represented in some metaphases. Eight markers are identified: del(1)(q21), der(1)t(1;2)(p36;q21), ?tdic(1;12)(p36;q24), ampl.t(4pter--->4q11::HSR::4q12--->4q24::HSR?::4q26--->4q35::2q15--->2pter), 13p+, 13p++, 14p+, t(14q15q), iso(14q), 19q+, del(3)(p14p24), 15p+.
Clinical Data 52 years Caucasian male
Tumorigenic Yes
Effects Yes, in nude mice; forms large cell carcinoma consistent with hepatoma
Comments
The SK-HEP-1 line has been identified as being of endothelial origin.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing 1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 10,13
D7S820: 8,11
THO1: 7,9
TPOX: 9
vWA: 14,17
Isoenzymes AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 1-2
PGM1, 2
PGM3, 1
Name of Depositor G Trempe, LJ Old
Year of Origin 1971
References Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
Heffelfinger SC, et al. SK HEP-1: a human cell line of endothelial origin. In Vitro Cell. Dev. Biol. 28A: 136-148, 1992. PubMed: 1371504
Gucev ZS, et al. Evidence for insulin-like growth factor (IGF)-independent transcriptional regulation of IGF binding protein-3 by growth hormone in SKHEP-1 human hepatocarcinoma cells. Endocrinology 138: 1464-1470, 1997. PubMed: 9075703
Turner BM, Turner VS. Secretion of alpha1-antitrypsin by an established human hepatoma cell line and by human/mouse hybrids. Somatic Cell Genet. 6: 1-14, 1980. PubMed: 6245472
