DavidCui 发表于 2016-9-24 16:54
尽快有公司将其进行ELISA试剂盒生产并应用。

抗原试剂盒要考虑到与登革及乙脑病毒的交叉

尽快有公司将其进行ELISA试剂盒生产并应用。

Structural Basis of Zika Virus-Specific Antibody Protection

Haiyan Zhao7, Estefania Fernandez7, Kimberly A. Dowd, Scott D. Speer, Derek J. Platt, Matthew J. Gorman, Jennifer Govero, Christopher A. Nelson, Theodore C. Pierson, Michael S. Diamond, Daved H. Fremont

Highlights
•New ZIKV-specific monoclonal antibodies are identified
•Three distinct epitopes on E protein DIII are defined by X-ray crystallography
•DIII lateral ridge antibodies broadly neutralize ZIKV infection and protect in mice

Summary
Zika virus (ZIKV) infection during pregnancy has emerged as a global public health problem because of its ability to cause severe congenital disease. Here, we developed six mouse monoclonal antibodies (mAbs) against ZIKV including four (ZV-48, ZV-54, ZV-64, and ZV-67) that were ZIKV specific and neutralized infection of African, Asian, and American strains to varying degrees. X-ray crystallographic and competition binding analyses of Fab fragments and scFvs defined three spatially distinct epitopes in DIII of the envelope protein corresponding to the lateral ridge (ZV-54 and ZV-67), C-C’ loop (ZV-48 and ZV-64), and ABDE sheet (ZV-2) regions. In vivo passive transfer studies revealed protective activity of DIII-lateral ridge specific neutralizing mAbs in a mouse model of ZIKV infection. Our results suggest that DIII is targeted by multiple type-specific antibodies with distinct neutralizing activity, which provides a path for developing prophylactic antibodies for use in pregnancy or designing epitope-specific vaccines against ZIKV.

http://www.cell.com/cell/abstract/S0092-8674(16)30927-8