Handbook: strategies for protein purification

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GE 公司上的资料,值得参考
Strategies for Protein Purification is focused on chromatographic purification of proteins atresearch scale. Beginners can use the handbook to get a quick start when entering the field;experienced protein purifiers may also find valuable information.The handbook describes how to design a protein purification procedure, how to select and combinechromatography methods and purification formats, and how to monitor and evaluate proteinpurification. It gives an overview of the available methods and provides advice on how to avoidpitfalls in all operations, from initial sample preparation to final analysis of the purified protein.The handbook is a complement to the series of handbooks from GE Healthcare that describe thedifferent chromatographic purification methods in more detail. The Recombinant Protein PurificationHandbook, another handbook from GE Healthcare, gives a large number of detailed protocolsfor the purification of recombinant proteins. For further reading on the various purificationmethods, see the list of all handbooks in the "Related literature" section at the end of this book.Most purifications are today performed with affinity-tagged proteins. This greatly simplifiespurification of many proteins. It is, however, relatively common that the target protein is unstableunder the conditions used for purification, or it may be difficult to obtain in sufficiently pure form.In such situations, more thorough purification protocol development may be needed to establishsuitable conditions for purification of active protein. Not all proteins to be purified are affinitytagged. Sometimes proteins are overexpressed without a tag because the tag would interferewith subsequent use of the protein. In other cases proteins from natural sources are purified.Under these circumstances, several chromatographic purification steps are usually required toobtain sufficient purity. This handbook describes a strategy for development of such protocols

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