Extracting Viral RNAs from Plant Protoplasts

donggua

endosome发表于 2007-10-21 14:04
http://biosky.haotui.com/thread-1190-1-3.html

Wiley 出版社2007年9月出炉。 The analysis of viral RNA is a fundamental aspect of plant RNA virus research. Studies that focus on viral RNAs often involve virus infections of plant protoplasts (see units 16d.1-16d.4). Protoplast offer the advantage of simultaneous initiation of infections, which allows for superior temporal and quantitative analyses of viral RNAs. The efficient isolation of intact viral RNA is key to any such investigations. This unit describes two basic protocols for extracting viral RNAs from plant protoplasts. An approach for preparing double-stranded viral RNA from total RNA pools is also provided. The viral RNA prepared by using these techniques can be used for further analyses such as primer extension, reverse transcription–PCR, and northern blotting

abbr_3830cf43e52c0be73baf355fe5cce8bd.pdf (65.79 KB)

donggua

randomqd回复：
从原生质体？

还以为是从叶绿体当中。。
去年我还想从叶绿体中提取RNA或者病毒粒子什么。。。
据说有提取细胞器的试剂盒