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本帖最后由 hantavirus 于 2017-3-30 23:51 编辑 : U: y* p, K k1 h4 X5 C
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演讲题目：Structure of viruses
+ \/ Z2 k! B4 c: @9 {演讲者：Prof. Vincent Racaniello, Ph.D.
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( C4 p* J ]3 |9 ?2 v/ i ?! O演讲者单位：哥伦比亚大学# D& i {- S0 d7 S
内容简介：主要讲解了下病毒的结构学相关知识，图文并茂，适合中级进阶者学习专业知识和练习英文之用。
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附完整视频：: x' x3 o$ G' R% F4 ?; W; B
总时长66分钟，1080高清，总计约280M，强烈建议在wifi环境下打开观看，土豪请随便！
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精彩内容展示：
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首先讲解了病毒结构蛋白的主要功能，主要有两个功能，即Protection of the genome和Delivery of the genome。: }, l+ m# r+ F; G
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, N" K2 p: M6 r9 B$ G. h/ `随后是阐述了关于病毒结构的几个重要概念，即：! d' n# K/ ?3 P, @$ p
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: p) w) K! Y6 K8 P) E The tools of viral structural biology
8 A; ?! `" D7 P2 V5 y+ i# w$ F• Electron microscopy
" B# e- j& O/ {1 d• X-ray crystallography
; R8 z6 ~9 E; Y5 N9 z; c• Cryo-electron microscopy (cryoEM) & cryo-electron tomography5 ^: N2 L+ k. ^, ~
• Nuclear magnetic resonance spectroscopy (NMR)! k, u% K7 w5 c
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展示了几种常见病毒的模拟结构彩图，如Poliovirus，Zika Virus等。
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Building virus particles: Symmetry is key
( d. ^8 S. F) f5 IWatson and Crick did more than discover DNA structure$ Y1 n9 t# u% i3 |- Y4 \# ] ^6 b
Their seminal contribution to virology:, J1 L3 U1 Q2 A- x) ]; q6 M0 y
- Identical protein subunits are distributed with helical symmetry for rod-shaped viruses. t3 X& T9 u4 ?& q2 o' B; t
- Platonic polyhedra symmetry for round viruses
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! g, L- P6 X8 p4 p1 `The symmetry rules are elegant in their simplicity，They provide rules for “self-assembly”3 K' }6 M/ o) B8 F9 j; X8 H
Rule 1: Each subunit has ‘identical’ bonding contacts with its neighbors
8 o& j% G. r, X1 g$ T; r& Z- Repeated interaction of chemically complementary surfaces at the subunit8 V. B7 i1 f& h1 c
interfaces naturally leads to a symmetric arrangement* L5 I. v# H) u) [
Rule 2: These bonding contacts are usually non-covalent
" R* c$ X- U) Y/ a- Reversible; error-free assembly
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% i+ J% u1 ~! N2 ^9 E5 pHow can you make a round capsid from proteins with irregular shapes?
, L, Y7 x. f" N- ]+ P# ]• Clue 1: All round capsids have precise numbers of proteins; multiples0 e6 U- s- a$ J2 K( s
of 60 are common (60, 180, 240, 960)
/ e/ e! d% j( v2 J* e• Clue 2: Spherical viruses come in many sizes, but capsid proteins are b5 v/ s; k" r I! i4 b
20-60 kDa average+ n* U0 f$ q3 R( m0 u9 @- d0 P
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附 Vincent R. Racaniello教授简介
$ y% M( K& e2 b) u Prof.Vincent R. Racaniello (born January 2, 1953 in Paterson, New Jersey) is a Higgins Professor in the Department of Microbiology and Immunology at Columbia University’s College of Physicians and Surgeons. He is one of four virologists who has recently authored Principles of Virology, a textbook used by many teaching virology to undergraduate, medical and graduate students.* g0 b) w, C0 N9 J$ ^1 Z3 ^
As an esteemed member of the scientific community, Racaniello has received several awards including Irma T. Hirschl, Searle Scholars, Eli Lilly and NIH Merit. He has also been a Harvey Society Lecturer at Rockefeller University, the Hilleman Lecturer at the University of Chicago, and University Lecturer at Columbia University. Racaniello has served on the editorial boards of scientific journals, including the Journal of Virology, and is a community editor for the open access journal PLOS Pathogens. He also served as the 2015 president of the American Society for Virology.
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