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本帖最后由 序道 于 2015-2-3 19:21 编辑 ; ^3 ]5 ?8 ^% n, `. m' h7 K
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IMMUNOLOGY% B5 m0 g2 I6 X" E8 m
60.401: g- i5 N0 v8 Q- _
LAB MANUAL 20046 ~* ~/ a/ P/ ], T( R
不错的书,下面是目录 : J! T/ d* b6 [+ P+ p2 H. R( A1 k- R
SCHEDULE 2; N$ P) O# B$ s. n2 L6 j; u0 O: z2 p
GENERAL INSTRUCTIONS 4 # _3 X# f1 \1 K
REFERENCE BINDER CONTENTS 8
* t4 R) N6 U2 c- E4 X! d+ F, ]STANDARD OPERATIONS PROCEDURE 9
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Lab 1: Preparation of Crude IgG by Salt Fractionation 13" p/ H! J) c8 n
Lab 2: Immunoprecipitation 17
7 N9 t3 h5 o% E( B; A/ E. qLab 3: Hemagglutination Assay 28
' ], L7 L# y O G9 f8 L$ fLab 4: Introduction to the Enzyme Linked Immunosorbent Assay (ELISA) 33
, ?5 D; L5 S' @" o* t- }8 G2 aLab 5: Part I: Induction and characterization of a primary cytokine 38$ ~! o! l3 Q" \% l/ h
response in vivo.
. R! |: h9 Q. h3 L+ MPart II: ELISA precision training.
0 c1 J* p+ V B% D! R" VLab 5: Part III: ELISA ASSAY- Quantitative analysis of IL-13 Response 477 n1 L# o* [5 d. ?# h# d$ c, _
Appendix:& j5 F* E8 L- c: x
Solutions 53! j) L9 O1 I3 F1 b- _- F
Floor Model Centrifugation Operation 54. |1 M: z% ~2 n9 d# F! C
Pipetman Operation 56
7 W4 |) p6 H6 f4 ?3 \, @Electrophoresis 58- r8 Z# t+ S7 r9 d
Sample Lab Exam 60 |
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