|
Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)0 o0 K4 c$ o& m& J$ X# J
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
m3 {% S1 l! B- C7 U, A( N; w
! o: T: ?/ C6 g5 WAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.! F. A7 q) Y7 k7 K
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little
$ `+ M. o- A& ?" {4 E& g9 \information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a9 _. D8 ]% f3 q$ n
key role in controlling viral infections. In this study, we report the cloning, expression, and6 ?( K/ J! ~) U$ f8 {9 E
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We' Y2 t2 {$ Z+ N+ b
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
6 B, ?# `/ F8 Q7 B; _* OM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant! B- w/ c' [5 v, ]& Y% F5 ^
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat U2 J# o% U1 A$ E3 y4 P. v1 c
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral6 X" c8 a& `% Y) [+ H1 J/ p
activity in microbats, which has important implications for virus interactions with these hosts.
& e- \5 P# ]/ \4 R0 W9 j
0 e5 P( Y' r6 J6 s9 `Keywords: bat; interferon; IFN-stimulated genes; antiviral activity
! V' Y( C7 c1 ?$ d
0 Y) K; L: d5 o2 w- _- H9 d
- ]# U/ y4 I: T% F" r1 }7 F$ Z8 ?中文信息:' ~& B& _! V+ D: n3 T
% @8 f' ~8 z. [: W- _% V- e
大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测4 C- f5 }' l& |0 R' C9 S
" P5 ?6 c+ p0 P& {- ~. \8 r/ D' l" ?# l$ u4 x7 O }8 V
梁英子,吴利军,张倩,周鹏,王媚娘,杨兴娄,葛行义,王林发,石正丽*
( ]) |' a5 H4 ~* [5 |* J2 K" z! F# o& M9 B6 K
9 i2 X0 m5 q" M! p3 W: P
摘要:蝙蝠是多种病毒的自然宿主,其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状,其中机制未知。近期对两种蝙蝠基因组序列分析结果提示,蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上,用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平;表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白,测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用,试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。$ a, V+ s# k/ V# Z0 i* N
: x: P* |2 ~/ Y" P* s
5 ^2 ]2 r+ ?/ ] O; w$ D# H关键词:干扰素,蝙蝠天然免疫,抗病毒活性,定量PCR
4 b- F' T8 o5 t* d* E
5 V4 d) U% D& p6 s/ a- j* q |
|