|
Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF), m0 m. |4 O4 G! n
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
8 _" s' Z% g( E! i
: r; x$ c- L2 q, Z) a% j7 kAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.$ A1 r( I+ l3 [
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little5 Q1 V1 T6 Z, t4 B
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
# W$ C! b! ?- S* h' ^key role in controlling viral infections. In this study, we report the cloning, expression, and
+ m% @) a- F4 }3 ubiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We$ ]0 A$ n( U1 n! o$ ~
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
; k: D& S, E) JM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
4 L) q$ N2 c' n) q( K' SIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat4 [2 V* Q+ M3 }7 y- S
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
& F% l( n7 a; W3 D; J* Sactivity in microbats, which has important implications for virus interactions with these hosts.! O7 V8 s" A5 |- H
3 U2 f) Q' \( T% s Z K* T. j7 N
Keywords: bat; interferon; IFN-stimulated genes; antiviral activity
3 u2 }# o. T: u$ B$ V+ r* N
/ k' o; L' M6 @& V, T4 z$ _, V ]: ?1 ]# Z
中文信息:
% a9 x1 q p* h* s& U- S: A9 S# [* H* O
大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
2 e4 Y' e; ]3 A9 |/ U$ v2 N# L8 P
) h' w0 f" ?9 r! s' i2 p梁英子,吴利军,张倩,周鹏,王媚娘,杨兴娄,葛行义,王林发,石正丽*& X% a* h9 L/ ` t4 @, ]% B
4 `: E: x! w h9 H r6 H/ n
3 A8 p% N! v" C) E- G摘要:蝙蝠是多种病毒的自然宿主,其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状,其中机制未知。近期对两种蝙蝠基因组序列分析结果提示,蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上,用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平;表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白,测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用,试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。3 L( i: p/ G9 Z0 S& J& j
; x* O9 a+ p( M7 [. J
3 B7 s1 \6 Z# ~5 U+ F! W( K6 {关键词:干扰素,蝙蝠天然免疫,抗病毒活性,定量PCR
/ [: e7 ~+ `: `. f+ Y8 E0 m
4 |+ e5 z% K0 O) F1 ~ |
|