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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)5 Q5 S. k2 y; U' N$ u/ F
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi** S- d: g0 @) Z$ W6 ]/ m2 h% m6 s$ O
( [2 ? Q" G2 Q0 i: t3 k( ]5 GAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.8 k1 n8 |. ?: c: y0 ^8 F' u
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little8 R/ p/ v1 j8 ~' X/ k2 _3 Y$ u
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
4 f3 }6 z+ [ p& \7 F" ^ G- p" nkey role in controlling viral infections. In this study, we report the cloning, expression, and7 I: l( N+ F& F3 |& l; i' H: ^
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
6 r$ P3 R" w. O5 ` M: ddemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from8 J! z4 w% U$ V8 K7 c G
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant; y2 Z* \1 \4 M B" w5 B
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat9 m% G4 m' l" S! i( e4 K$ v7 O6 X G
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
6 |; Z, C: c! i' i2 mactivity in microbats, which has important implications for virus interactions with these hosts.
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Keywords: bat; interferon; IFN-stimulated genes; antiviral activity2 R$ {, r1 g: ~7 n9 o% K4 w
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大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测, b% V1 D" W) S: a& v
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梁英子,吴利军,张倩,周鹏,王媚娘,杨兴娄,葛行义,王林发,石正丽*
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摘要:蝙蝠是多种病毒的自然宿主,其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状,其中机制未知。近期对两种蝙蝠基因组序列分析结果提示,蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上,用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平;表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白,测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用,试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。$ I! U J6 ^& f6 I: a, V: m$ V" F7 ?
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" h1 L/ W" M6 C' f3 m$ B( L8 x关键词:干扰素,蝙蝠天然免疫,抗病毒活性,定量PCR# v# \# l5 C5 Z% ~# ?+ R* z
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