禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
' F6 j: ^2 X' {1 n) G" U【作    者】：Erica Spackman Editor
- @6 r0 r- C& s! w9 X: H1 i【出 版 社】: Humana Press
6 c$ {/ p( H- I【简    介】：
* B8 W0 `2 o' D2 Q. }   08新书,大家共享    附件PDF下载！
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, L* u8 V! P) ^' D4 Y3 C   The aim of this book is to create a resource that includes both basic methods that
are currently used and well established as well as some of the most exciting new
methods for studying the virus itself, and to include methods that focus on work
7 r6 j' E! Z5 R% U0 R6 \with avian hosts, an area that has been greatly lacking. In that context, this volume
will be of interest to both diagnosticians and researchers, but it does assume a basic
, L5 @% f! H# o2 v  U- H+ y, Vknowledge of virology and molecular biology.
/ E& ]7 J$ t+ C' eI would like to thank all the authors for their contributions. I am also thankful to
John M. Walker, the series editor, for his help in editing and working with me
/ W# N, _; D9 h' ?& S) {8 N- P  h/ Ithrough this project.
Erica Spackman
3 v" x, r9 M+ P/ K( s# F7 ~Athens, GA, September 2007
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( |$ \6 n% b0 i7 }9 `* l' q【出版日期】：2008
& \, }5 F8 z( Q. }: c1 t+ [! G【目    录】：
9 y# F. b6 @! S: `% kPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
Erica Spackman
1 t% ~4 i4 U+ `/ o, O5 \' w; N2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
Mary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
) O/ N8 G" Q9 a. Oand Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
1 H/ b( M6 X3 M- g7 eErica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
& ^9 ]2 K2 s1 M0 y% m6 D* W( R4 \by Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
' J: Q7 O) w! R) z) W: p4 m6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Peter R. Woolcock
7 R5 Y& X4 ?4 R- B$ s7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
& {) C3 n+ |. ~6 MMary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
( F  H( I8 T- n# XSubtype Identifi cation and the Detection and Quantitation
of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
( t  ^& ]8 M+ p) I8 lJanice C. Pedersen
8 _) W% z5 n4 x. _* Pvii
5 j) L) Q0 R, X' J% {& a9 Neuraminidase-Inhibition Assay for the Identifi cation
of Infl uenza A Virus Neuraminidase Subtype or
3 V! s2 E" P: ^6 D/ P. n" pNeuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Janice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
1 Y$ k# f2 U2 }) H+ ^% qAvian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Mary J. Pantin-Jackwood
! q' f$ H2 i0 R, V0 D11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
* Q2 \0 C# M* U3 }Chang-Won Lee and David L. Suarez
13 Evaluating the Cell-Mediated Immune Response of Avian
Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
' N; n/ ~7 A2 C2 t: sDarrell R. Kapczynski and Michael H. Kogut
2 n# @% B3 ?/ x, `8 u7 q# sIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
6 `0 Y. U2 l- ?; z+ yviii Contents

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