禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
( x' E! h9 f& V; Q1 \【简    介】：
1 n3 C9 {9 }2 ]! ]/ o0 Z6 N/ G   08新书,大家共享    附件PDF下载！

$ M- b" i$ ~7 E. k8 _   The aim of this book is to create a resource that includes both basic methods that
4 _: d) g2 L* j% T* D2 P) oare currently used and well established as well as some of the most exciting new
methods for studying the virus itself, and to include methods that focus on work
8 T5 i  K4 u1 V7 D9 Xwith avian hosts, an area that has been greatly lacking. In that context, this volume
will be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
  ~+ s3 V. a4 xI would like to thank all the authors for their contributions. I am also thankful to
# f* i3 t+ U. {2 T( |John M. Walker, the series editor, for his help in editing and working with me
through this project.
9 p% _  c* K* l0 I0 CErica Spackman
% `0 {) I$ U% h9 K3 N6 B2 V+ I# WAthens, GA, September 2007

【出版日期】：2008
【目    录】：
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
Erica Spackman
" P9 J% K. {, \; O/ E1 ~5 D2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
5 C: G' D6 o3 aMary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
+ T1 F8 m/ U' C- VErica Spackman and David L. Suarez
+ y& [0 c, J! j+ d3 G  p: `) {4 Type A Infl uenza Virus Detection and Quantitation
, {6 r5 F( M5 K& j- m+ ]2 o8 K& Xby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
* L, R( B3 w' o; z5 B5 Detection and Identifi cation of the H5 Hemagglutinin
Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
6 Avian Infl uenza Virus Isolation and Propagation
- H2 P, D. Q; r8 D7 ]. win Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Peter R. Woolcock
% n4 G* K# H; i0 Z- g7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
  P2 Z* y0 m* ?8 ~) c" Z5 OMary Lea Killian
+ M3 t* b) i( ?( U. l! S- r8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
Subtype Identifi cation and the Detection and Quantitation
$ r8 |- o1 j4 i7 oof Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
Janice C. Pedersen
vii
9 Neuraminidase-Inhibition Assay for the Identifi cation
5 ^# V, a+ d6 p& k8 H$ ]% F; Iof Infl uenza A Virus Neuraminidase Subtype or
# J1 ]6 V3 i& O8 `! [Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Janice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
( y, H( Z6 F" X: _5 x1 VAvian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Mary J. Pantin-Jackwood
11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
; j! s! i- g# w3 f/ o, oChang-Won Lee and David L. Suarez
13 Evaluating the Cell-Mediated Immune Response of Avian
" Y+ Y! l/ `$ F$ TSpecies to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
# J! W% r. t( J7 ?- h) j: iFollowing Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
4 O* C; m7 I+ T! w! cIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents


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