禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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本帖转自 http://biosky.haotui.com/viewthread.php?tid=6402  作者：pyau
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5 {# b7 s; ~/ A8 u7 p) L. S. U6 I08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
6 r. K  P0 [' P$ A& x【作    者】：Erica Spackman Editor
/ z0 h: Z$ l! U; H【出 版 社】: Humana Press
【简    介】：
   08新书,大家共享    附件PDF下载！
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   The aim of this book is to create a resource that includes both basic methods that
are currently used and well established as well as some of the most exciting new
* q0 K8 l( J- |4 T1 L8 ?methods for studying the virus itself, and to include methods that focus on work
" \8 y2 s# v, J3 h" g7 iwith avian hosts, an area that has been greatly lacking. In that context, this volume
will be of interest to both diagnosticians and researchers, but it does assume a basic
5 F7 O+ |: U' q# L  g9 J( Gknowledge of virology and molecular biology.
* Z" D( h1 Q6 D- ~% D+ H  ]I would like to thank all the authors for their contributions. I am also thankful to
John M. Walker, the series editor, for his help in editing and working with me
5 A: q& P% o6 R$ sthrough this project.
1 M- @/ r3 }: ^: KErica Spackman
Athens, GA, September 2007
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【出版日期】：2008
' y) p& D3 o0 z4 |9 J【目    录】：
8 y% G; {7 ~/ U- zPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
+ Q; W. j; v: {. p* @" b; M: cContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
' D) N2 m* C# y0 dErica Spackman
8 c& }+ G( a! h' K( R0 ~$ C2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
; m, }# w/ O# S$ Y; E3 tMary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
. @: N0 h' u: S2 X& K' ~! Oand Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
0 W" H  Q3 G7 t4 Type A Infl uenza Virus Detection and Quantitation
by Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
; k+ G3 d, U2 V( n: s4 lErica Spackman and David L. Suarez
6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Peter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
. x1 x& h. r4 x1 j1 t1 aMary Lea Killian
/ T  e/ V8 U8 v: @! ?' _& S, C8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
8 f0 K; k4 }5 L) D; mSubtype Identifi cation and the Detection and Quantitation
of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
Janice C. Pedersen
8 R- v$ Z/ }9 Y+ m( w7 [& S. Yvii
9 Neuraminidase-Inhibition Assay for the Identifi cation
7 @: {, N. m: n3 U- Jof Infl uenza A Virus Neuraminidase Subtype or
Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Janice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
" c0 C7 x8 h: D& z& x4 e7 M9 iMary J. Pantin-Jackwood
. @" a2 I( B* U7 O11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
) B- Z; u  \+ b" }% B5 \Justin D. Brown and David E. Stallknecht
0 B3 O0 r4 ]0 @# S; [- \9 ^/ Q12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
13 Evaluating the Cell-Mediated Immune Response of Avian
" Z0 D2 Y- b+ Z0 U/ {Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
/ j- g8 A7 b% RDarrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
2 I6 P9 V) A% C7 I! F4 V  {3 x5 Fviii Contents
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