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【书名版次】: Adeno-Associated Virus: Methods and Protocols (Methods in Molecular Biology)
! p( n& j4 P+ o u3 ^1 h/ `. R【作 者】:Richard O. Snyder (Editor), Philippe Moullier (Editor)
! u& a5 E( k1 ]" W【出 版 社】: Humana Press; 2011 edition; D( P2 q) P* o
【出版日期】: (November 30, 2011) 0 y% I0 C& x" r6 o$ n) Q
0 I; u% ?1 V4 j5 ?【简 介】: Today, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding — or at least an appreciation of — the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Protocols, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Adeno-Associated Virus: Methods and Protocols provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.; Q. u' N4 c4 ?& V3 D
【目 录】:
9 A- o) ~6 q7 ~! o1 s DHardcover: 475 pages; @$ k0 @+ ?7 U/ X" H
Language: English: }9 _# V! I- T) A% ~; k
ISBN-10: 1617793698
$ e' f- I( s" @ x- B5 n- i( x" @ISBN-13: 978-1617793691( _$ m6 S$ H7 R0 W
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