|
|
Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)
5 x( x2 ~! _: h0 t3 y( MYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
2 `$ R' A4 v( T% ?; |
5 B. |' \$ l! X2 @5 IAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.' P" e) l- t8 n* ~6 Y8 E
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little" {: p% f- u4 h' e9 R
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
4 p9 |7 |1 j& H3 E- C* Kkey role in controlling viral infections. In this study, we report the cloning, expression, and6 q9 L% {+ m5 x- m
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We; p8 |: i4 H; q6 w. X$ f8 l
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
+ s$ [9 N* `5 k& \5 H5 e1 V% C1 hM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
: P' S8 k! l1 R7 a1 L' X* s( DIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
" f$ x9 [5 d: F8 t5 C& lspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral0 o1 m* c$ F& K9 x' z
activity in microbats, which has important implications for virus interactions with these hosts.' F7 ~& ^+ C, O0 `+ i! w* F+ Y9 I
, g( u, N9 s' T% S9 k5 cKeywords: bat; interferon; IFN-stimulated genes; antiviral activity! D* u' `3 |( J5 n+ v- l
! N8 Z# O5 Z: P3 g+ P. @6 Y5 N; c1 f9 H! w& Q( w% a) P/ f+ m, ^# \
中文信息：
( Q+ D2 z- ^4 f* l r! h0 e+ e5 W+ B6 S: x, O
大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测* q2 [7 A7 T7 s, T z1 ?
% b, A4 }- W! w2 j' Q7 t" j) ~9 j" }- h
梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
5 v1 N8 H0 Z$ Q, E- [ I' m3 D8 ^* T/ ]- x n4 H. O
* \ o. h6 U/ u) A, S; `# g. e摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。8 _* U- J! ^7 W2 c' e% l P t8 o
, \5 H! t! C) q4 ?8 k }6 C
9 J: z3 ]) b- S( v6 }% g关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR6 K& f7 B- H( L$ s+ }6 _
0 `% w' d3 G9 Q4 d. p& I( T+ B |
|
发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
