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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
! Y3 k; r6 z( a9 F* FYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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# o; ^- N/ j5 F( L: o* H; NAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
. M  x3 Q2 w- i- sTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
! L/ W- ^: W" _; A/ Minformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
  F7 [+ Y; S5 ^+ n- i+ okey role in controlling viral infections. In this study, we report the cloning, expression, and
& U& U9 Q, u9 S$ \1 v- S) ~biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
+ w% b" @- H. z+ s9 zdemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
& T* |/ b" Y8 n  o/ R2 I. A4 C9 YM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
+ X- O. I5 _: e3 z+ J+ r" `# wspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
% p& [* h3 h$ a# Z+ F$ \activity in microbats, which has important implications for virus interactions with these hosts.

Keywords: bat; interferon; IFN-stimulated genes; antiviral activity
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大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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! e! Y8 O, Y1 O" Y$ f/ p梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*


6 J6 S. Y5 z3 R& e摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
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8 t' y6 J8 V% Q0 X4 H  ^关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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