cell host&microbe:华人科学家成功克隆出zika，加速疫苗研发

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美国得克萨斯大学华人科学家史佩勇研究组最近成功克隆出寨卡病毒，这项成果有助加速寨卡疫苗的研发。

研究人员在新一期美国《细胞宿主与寄生体》杂志上发表报告说，他们选取了2010年分离自柬埔寨的毒株进行克隆。根据其基因组序列，先把病毒基因组分成5个片段，然后分别克隆这些片段，再将克隆产物组装成一个完整的病毒基因组，接着把克隆的基因组通过分子生物学的方法导入细胞，从而获得克隆的病毒。

小鼠实验显示，克隆病毒可以导致小鼠患病，它们会出现一些神经系统疾病的症状，这跟寨卡病毒可以引起人类的神经系统疾病类似。

研究人员还做了克隆病毒的蚊子传播实验。结果显示，克隆病毒有着类似于母系病毒的传播效率。

研究人员表示，克隆寨卡病毒有助于对其有更加深入的了解，比如可以对任意位点、任意蛋白进行突变，从而了解寨卡病毒的致病机理以及哪些蛋白对于致病发挥重要作用。

史佩勇研究组接受新华社记者电子邮件采访时说：“克隆病毒的获得对于我们加速对寨卡病毒的认知提供了一个很重要的工具，将会加速对寨卡病毒的基础研究和应用研究。我们希望能与各个实验室广泛开展合作。目前我们正在积极推进与中国一些课题组的合作。”

来源：新华社

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An Infectious cDNA Clone of Zika Virus to Study Viral Virulence, Mosquito Transmission, and Antiviral Inhibitors

Chao Shan, Xuping Xie, Antonio E. Muruato, Shannan L. Rossi, Christopher M. Roundy, Sasha R. Azar, Yujiao Yang, Robert B. Tesh, Nigel Bourne, Alan D. Barrett, Nikos Vasilakis, Scott C. Weaver, Pei-Yong Shi

Highlights
•An infectious cDNA clone of Zika virus and a luciferase reporter virus were developed
•Recombinant Zika virus is virulent in A129 and AG129 mice
•Recombinant Zika virus is highly infectious for Aedes aegypti mosquitos
•The luciferase Zika virus can be used for antiviral drug discovery

Summary
The Asian lineage of Zika virus (ZIKV) has recently caused epidemics and severe disease. Unraveling the mechanisms causing increased viral transmissibility and disease severity requires experimental systems. We report an infectious cDNA clone of ZIKV that was generated using a clinical isolate of the Asian lineage. The cDNA clone-derived RNA is infectious in cells, generating recombinant ZIKV. The recombinant virus is virulent in established ZIKV mouse models, leading to neurological signs relevant to human disease. Additionally, recombinant ZIKV is infectious for Aedes aegypti and thus provides a means to examine virus transmission. The infectious cDNA clone was further used to generate a luciferase ZIKV that exhibited sensitivity to a panflavivirus inhibitor, highlighting its potential utility for antiviral screening. This ZIKV reverse genetic system, together with mouse and mosquito infection models, may help identify viral determinants of human virulence and mosquito transmission as well as inform vaccine and therapeutic strategies.

http://www.cell.com/cell-host-microbe/abstract/S1931-3128(16)30200-1

cao1976

学习了

ghx0123

呵呵，刚还在搜索原文，准备明天在微信公众号上发表，就在论坛看见原文了，拿走不谢

zhaox

非常不错，学习了好多。

wx_FbAxhwwB

原文在哪？链接打不开啊

ipsvirus

wx_FbAxhwwB 发表于 2016-5-20 15:57
原文在哪？链接打不开啊

应该是格式问题，没有把全部内容链接上，所以不要直接点击链接，全部复制到地址栏上打开即可。

langxiuyan

谢谢 分享