禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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. `. f/ Q3 L  x& W0 r8 }4 v+ h9 J& T08年新书 Avian Influenza Virus
; @" X: b5 }+ ~: C【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
) y' Y" u- E1 T/ o% D7 V【简    介】：
   08新书,大家共享    附件PDF下载！

  I3 f; I( P6 o# t+ i   The aim of this book is to create a resource that includes both basic methods that
are currently used and well established as well as some of the most exciting new
5 V& c$ G% I' u+ u: E3 [0 `8 z: Hmethods for studying the virus itself, and to include methods that focus on work
7 ?, V4 D; e- vwith avian hosts, an area that has been greatly lacking. In that context, this volume
5 S  j, F1 Y% D* {6 Kwill be of interest to both diagnosticians and researchers, but it does assume a basic
  f2 p5 @8 h) Y- Pknowledge of virology and molecular biology.
+ A3 z! @% _& @# ^( VI would like to thank all the authors for their contributions. I am also thankful to
John M. Walker, the series editor, for his help in editing and working with me
! M8 b( D9 O+ C4 {  d' o) ithrough this project.
2 t) H6 @1 i5 u% O/ ?# aErica Spackman
Athens, GA, September 2007
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【出版日期】：2008
【目    录】：
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
' ?2 C* A  n" q  b$ b7 b4 z0 \Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
. \; _& T! b& c1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
) R* u( G* g% o3 YErica Spackman
2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
Mary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
by Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
) g  ?: {" I; K4 cSubtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
) Q- l6 s# U# s. uErica Spackman and David L. Suarez
1 m( \% Z  w) Z/ c4 u6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
8 d+ e. m; S9 _8 I/ ^6 PPeter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
Mary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
/ R0 C$ o" t. U: q7 Y- ^+ P1 E6 @% XSubtype Identifi cation and the Detection and Quantitation
) a' v5 \/ G+ y& wof Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
$ T0 m+ }: M/ L* Y& a! e7 gJanice C. Pedersen
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9 Neuraminidase-Inhibition Assay for the Identifi cation
of Infl uenza A Virus Neuraminidase Subtype or
Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Janice C. Pedersen
! y; G3 q: D& n' @: ~4 ?7 D% E10 Immunohistochemical Staining for the Detection of the
Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Mary J. Pantin-Jackwood
+ B# H6 N! n' P6 e11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
. y; t- b: H- e& U1 o* j3 v+ @12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
: o/ t- z0 \, N4 G! q13 Evaluating the Cell-Mediated Immune Response of Avian
& q6 }# z0 s6 J  n0 QSpecies to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
$ H$ x( A' S" Y; R9 C" `9 w14 Measurement of Avian Cytokines with Real-Time RT-PCR
- O- o/ W% Y7 d5 _+ ?Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
4 \( B- j. r+ W( [. x, `Darrell R. Kapczynski and Michael H. Kogut
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents

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