禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
" b5 Z! ]' n* F/ N$ F4 \【作    者】：Erica Spackman Editor
, j# L# p8 h' g8 R4 @- M) Z# m2 |【出 版 社】: Humana Press
: @' ]0 G& q& G3 ^7 N! w* C  A【简    介】：
   08新书,大家共享    附件PDF下载！
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   The aim of this book is to create a resource that includes both basic methods that
are currently used and well established as well as some of the most exciting new
( q+ n: u' O/ v" |2 umethods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
3 R0 j; v! d1 W# Hwill be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
I would like to thank all the authors for their contributions. I am also thankful to
John M. Walker, the series editor, for his help in editing and working with me
$ r3 n3 Y' R9 h' M* Lthrough this project.
Erica Spackman
Athens, GA, September 2007
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, p2 u8 V5 [. B6 `; d【出版日期】：2008
( Z+ U& b' f1 Q0 _% a. U5 A' G【目    录】：
. {0 [) Y% ^7 u$ H2 QPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
5 \. K+ `5 c& ~3 @5 rContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
2 a- r1 w& T& b6 E! JErica Spackman
( {/ m4 X+ Y% p8 V1 j/ M' G3 d2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
Mary Lea Killian
: z: e! @" ], Q, @; ^3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
% {4 Z: a$ H7 E7 R7 Q  tby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
& N8 V: C7 v9 B% f' O; V9 ^5 Detection and Identifi cation of the H5 Hemagglutinin
Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
; a3 L2 q9 l; s# r6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
7 I6 D' a" w7 G% l6 dPeter R. Woolcock
2 u' N' L! U7 ]+ z& n  n7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
  l+ R" V. |& xMary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
Subtype Identifi cation and the Detection and Quantitation
of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
Janice C. Pedersen
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9 Neuraminidase-Inhibition Assay for the Identifi cation
of Infl uenza A Virus Neuraminidase Subtype or
. h3 v% ~' ]* K% Q# @; XNeuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Janice C. Pedersen
* Q$ F2 \8 ?( M0 k' i6 [* |10 Immunohistochemical Staining for the Detection of the
Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Mary J. Pantin-Jackwood
- P$ O/ J* |* I9 R5 a11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
4 m- ]) n! ?- ^, I. ~0 zChang-Won Lee and David L. Suarez
# A: w1 f/ V$ P3 j  o: W$ z% C13 Evaluating the Cell-Mediated Immune Response of Avian
% ^- r# Q, f4 n; E- [  ^0 c6 ASpecies to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
! Y, B8 Q% G7 LDarrell R. Kapczynski and Michael H. Kogut
% A! ?0 I9 E2 l# h& O% |Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
* |% l! G0 W$ I! v; b+ Fviii Contents


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