禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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08年新书 Avian Influenza Virus
! I. Z% g% K: d【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
【简    介】：
   08新书,大家共享    附件PDF下载！

   The aim of this book is to create a resource that includes both basic methods that
are currently used and well established as well as some of the most exciting new
  o( d6 u$ `5 N. m- V' Omethods for studying the virus itself, and to include methods that focus on work
# e! [! e' g9 u. N; J' gwith avian hosts, an area that has been greatly lacking. In that context, this volume
% I1 s' E# E1 Y; U6 Awill be of interest to both diagnosticians and researchers, but it does assume a basic
* E' t$ ~: O9 h4 d% N( Q% Oknowledge of virology and molecular biology.
: l8 [5 Q/ X7 e0 i; _' [* R6 FI would like to thank all the authors for their contributions. I am also thankful to
' B4 G5 D% f; x5 gJohn M. Walker, the series editor, for his help in editing and working with me
( [. s5 o6 E/ `7 I4 Dthrough this project.
0 L) o* V% W# v% k1 j# g$ d6 M- ?Erica Spackman
- x9 I( c* n' {; ?* [  UAthens, GA, September 2007

【出版日期】：2008
0 O3 c) _/ h( w【目    录】：
9 Q5 h+ p! q/ p, pPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
5 u& g3 F$ k: h7 K3 aContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
/ w: T) F( l& X1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
" `# p$ G$ ?$ C2 H) TErica Spackman
2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
Mary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
. E8 m/ }* N9 j$ i$ M) EErica Spackman and David L. Suarez
4 d9 n' u6 i: {* ]- n0 U4 Type A Infl uenza Virus Detection and Quantitation
by Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
6 m# u4 Y1 d; S* a2 ~4 CErica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
( ]/ K) Q, r  b& V' vSubtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
7 w1 g- m+ ~, t; MErica Spackman and David L. Suarez
6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Peter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
Mary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
Subtype Identifi cation and the Detection and Quantitation
$ v3 ~& E6 a7 ~6 I' V; B4 fof Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
/ R. C% m* d9 \1 F! h/ bJanice C. Pedersen
vii
* d: y2 ~8 \4 {8 ~# M8 U; U+ l8 p% u9 Neuraminidase-Inhibition Assay for the Identifi cation
of Infl uenza A Virus Neuraminidase Subtype or
3 s, W/ A/ d  W& S  @' BNeuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Janice C. Pedersen
( L( Y( o1 k8 P$ m10 Immunohistochemical Staining for the Detection of the
% H2 _+ g+ X3 H5 `0 E0 y0 iAvian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
) |% y$ Z  F- I% f$ D6 GMary J. Pantin-Jackwood
. z1 T/ p" o9 M- Z0 U! c$ N11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
: z0 \1 h1 ]: d2 d12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
5 ^6 P2 V9 q2 I: GChang-Won Lee and David L. Suarez
* `, ^8 {, b, S8 n+ u13 Evaluating the Cell-Mediated Immune Response of Avian
Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
* ?1 Y# K7 ?7 i: X1 [" A14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
7 f( o0 @$ t+ Z6 v$ O! K6 e: W% JIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents

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