[转移帖]Francis V. Chisari scripps研究所——HBV相关实验室及人物介绍系列

cao1976

原帖由bigben发表于 2010-12-5 04:17 ：

1 简介
The Scripps Research Institute, Department of Immunology and Microbial Science, La Jolla, California, USA
我很少看他的文章，jane200988推荐的，不过是大牛是毋庸置疑的，看看他的文章列表就知道了，偏向于HBV免疫。

2 实验室链接
http://www.scripps.edu/research/faculty.php?rec_id=335

3 研究领域
Immunobiology and Pathogenesis of Hepatitis B and C Virus Infection

The outcome of noncytolytic viral infections is largely determined by the cellular immune response. Our laboratory focusses on the host-virus interactions that determine the outcome of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections. Using a combination of real time viral dynamics and quantitative functional T cell analysis, we have demonstrated a direct relationship between viral clearance and the vigor, kinetics and homing potential of the antiviral T cell response to HBV and HCV, the rate with which they spread after inoculation and their ability to escape immune recognition. In addition, we have shown that there is a third effector limb in the immune response besides antibody neutralization and T cell-mediated destruction of infected cells: i.e. T cells also secrete antiviral cytokines that can purge viruses from infected cells without killing them. Currently we are applying genome-wide expression profiling to define the intracellular antiviral effector pathways that are triggered by these cytokines; biochemical, biophysical and molecular approaches to identify the points in the life cycles of these viruses that are targeted by these antiviral cytokines; and molecular immunological strategies to determine the mechanisms deployed by the viruses to defeat their antiviral effects.

4 文章
太多了，去他主页自己看吧
McClary, H., Koch, R., Chisari, F.V., and Guidotti, L.G. Relative sensitivity of hepatitis B virus and other hepatotropic viruses to the antiviral effects of cytokines. J. Virol. 74:2255-2264, 2000.

Chisari, F.V.  Rous-Whipple Award Lecture.  Viruses, Immunity, and Cancer:  Lessons from Hepatitis B.  Am. J. Pathol. 159:1117-1131, 2000.

Wieland, S.F., Guidotti, L.G., and Chisari, F.V. Intrahepatic induction of alpha/beta interferon eliminates viral RNA-containing capsids in HBV transgenic mice. J. Virol. 74:4165-4173, 2000.

Schirmbeck, R., Wild, J., Blum, H.E., Chisari, F.V., Geissler, M., and Reimann, J. Ongoing murine T1 or T2 immune responses to the hepatitis B surface antigen (HBsAg) are excluded from the liver that expresses transgene-encoded HBsAg. J. Immunol. 164:4235-4243, 2000.

Guidotti, L.G., McClary, H., Moorhead, J., and Chisari, F.V. Nitric oxide inhibits hepatitis B virus replication in the liver of transgenic mice. J. Exp. Med. 191:1247-1252, 2000.

McClary, H., Koch, R., Chisari, F.V., and Guidotti, L.G.  Inhibition of hepatitis B virus replication during Schistosoma Mansoni infection in transgenic mice.  J. Exp. Med. 192:289-294, 2000.

Guidotti, L.G. and Chisari, F.V.  Cytokine-mediated control of viral infections.  In: Virology 273:221-226, 2000.

Pasquetto, V., Guidotti, L.G., Kakimi, K., Koch, R., Tsjui, M., and Chisari, F.V.  Host-virus interaction during malaria infection in hepatitis B virus transgenic mice.  J. Exp. Med. 192:529-535, 2000.

Voehringer, D., Blaser, C., Busse-Grawitz, A., Chisari, F.V., Buerki, K., and Pircher, H.  Break of T cell ignorance to a viral antigen in the liver induces hepatitis.  J. Immunol. 165:2415-2423, 2000.

Pasquetto, V., Wieland, S., and Chisari, F.V.  Intracellular Hepatitis B Virus nucleocapsids survive cytotoxic T-Lymphocyte induced apoptosis. J. Virol.74:9792-9796, 2000.

Kakimi, K., Guidotti L., Koezuka, Y., and Chisari F.V.  Natural killer T cell activation inhibits hepatitis B virus replication in vivo. J. Exp. Med. 192:921-930, 2000.

McKinney, D.M., Erickson, A.L., Walker, C.M., Thimme, R., Chisari, F.V., Purcell, R., Sidney, J., and Sette, A.  Identification of five different Patr class I molecules which bind HLA supertype peptides and definition of their peptide binding motifs. J. Immunol. 165:4414-4422, 2000.

Forns, X., Thimme, R., Govindarajan, S., Emerson, S.U., Purcell, R.H., Chisari, F.V., and Bukh, J.  Hepatitis C Virus lacking hypervariable region 1 of the second envelope protein is infectious and causes acute resolving or persistent infection in chimpanzees.  Proc. Nat'l. Acad. Sci. USA 97:13318-13323, 2000.

Guidotti, L.G. and Chisari, F.V.  Noncytolytic control of viral infections by the innate and adaptive immune response.  Annu. Rev. Immunol. 19:65-91, 2001.

Chang, K-M., Thimme, R., Melpolder, J., Oldach, D., Pemberton, J., Moorhead-Loudis, J., McHutchison, J.G., Alter, H.J., and Chisari, F.V. Differential CD4+ and CD8+ T cell responsiveness in hepatitis C virus infection.  Hepatology  33:267-276, 2001.

Sette, A., Oseroff, C., Sidney, J.,  Alexander, J., Chesnut, R.W., Kakimi, K., Guidotti, L.G., and Chisari, F.V.  Overcoming T cell tolerance to the hepatitis B virus surface antigen in Hepatitis B Virus-transgenic mice.  J. Immunol. 166:1389-1397, 2001.

Schirmbeck, R., Zheng, X., Roggendorf, M., Geissler, M., Chisari, F.V., Reimann, J., and Lu, M.  Targeting murine immune responses to selected T cell- or antibody-defined determinants of the hepatitis B surface antigen (hBsAg) by plasmid DNA vaccines encoding chimeric antigen.  J. Immunol. 166:1405-1413, 2001.

Thimme, R., Chang, K.M., Pemberton, J., Sette, A., and Chisari, F.V.  Degenerate Immunogenicity of an HLA-A*0201-restricted HBV nucleocapid CTL epitope that is also presented by HLA-B*5101.  J. Virol. 75:3984-3987, 2001.

Ferrari, C. and Chisari, F.V.  Immune mechanisms of viral clearance and disease pathogenesis during viral hepatitis. In: The Liver: Biology and Pathobiology 4th Edition.  Arias, I.M., Boyer, J., Chisari, F.V., Fausto, N., Schachter,  and Shafritz, D. (Eds.).  Lippincott, Williams & Wilkins, Philadelphia, PA, pp. 763-782, 2001.

Heise, T., Guidotti, L.G., and Chisari, F.V.  Characterization of nuclear ribonucleases that cleave hepatitis B virus RNA near the La protein binding site.  J. Virol. 75:6874-6883, 2001.

Thimme, R., Oldach, D., Chang, K.M., Steiger, C., Ray, S., and Chisari, F.V.  Determinants of viral clearance and persistence during acute HCV infection.  J. Exp. Med. 194:1395-1406, 2001.

Kakimi, K., Lane, T.E., Chisari, F.V., and Guidotti, L.G. Inhibition of hepatitis B virus replication by activated NKT cells does not require inflammatory cell recruitment to the liver.   J. Immunol. 167:6701-6705, 2001.

Kakimi, K., Campbell, I., Lane, T., Wieland, S.F., Chisari, F.V., and Guidotti, L.G.  Blocking chemokine responsive to g-2/interferon (IFN)-g inducible protein and monokine induced by IFN-g activity in vivo reduces the pathogenetic but not the antiviral potential of hepatitis B virus-specific cytotoxic T lymphocytes.  J. Exp. Med. 194:1755-1766, 2001.

Mosier, D.E. and Chisari, F.V.  GB and virus C and mortality from HIV infection.  N. Engl. J. Med. 346:377-379, 2002.

Guidotti LG, Morris A, Mendez H, Koch R, Silverman RH, Williams BR, and Chisari FV. Interferon-regulated pathways that control hepatitis B virus replication in transgenic mice.
J Virol. 76:2617-21, 2002.

Robek, M.D., Wieland, S.F., and Chisari, F.V.  Inhibition of hepatitis B virus replication by interferon requires proteasome activity. J. Virol. 76:3570-3574, 2002.

Pasquetto, V., Wieland, S.F., Uprichard, S.L., Tripodi, M., and Chisari, F.V.  Cytokine sensitive replication of hepatitis B virus in immortalized mouse hepatocyte cultures.

cao1976

yaoming发表于 2010-12-5 10:22 ：

Francis V. Chisari 现在做HCV了，大科学家换方向很容易啊！

1: Gastaminza P, Dryden KA, Boyd B, Wood MR, Law M, Yeager M, Chisari FV.
Ultrastructural and biophysical characterization of hepatitis C virus particles
produced in cell culture. J Virol. 2010 Nov;84(21):10999-1009. Epub 2010 Aug 4.
PubMed PMID: 20686033; PubMed Central PMCID: PMC2953183.


2: Takahashi K, Asabe S, Wieland S, Garaigorta U, Gastaminza P, Isogawa M,
Chisari FV. Plasmacytoid dendritic cells sense hepatitis C virus-infected cells,
produce interferon, and inhibit infection. Proc Natl Acad Sci U S A. 2010 Apr
20;107(16):7431-6. Epub 2010 Mar 15. PubMed PMID: 20231459; PubMed Central PMCID:
PMC2867703.


3: Bissig KD, Wieland SF, Tran P, Isogawa M, Le TT, Chisari FV, Verma IM. Human
liver chimeric mice provide a model for hepatitis B and C virus infection and
treatment. J Clin Invest. 2010 Mar 1;120(3):924-30. doi: 10.1172/JCI40094. Epub
2010 Feb 22. PubMed PMID: 20179355; PubMed Central PMCID: PMC2827952.


4: Garaigorta U, Chisari FV. Hepatitis C virus blocks interferon effector
function by inducing protein kinase R phosphorylation. Cell Host Microbe. 2009
Dec 17;6(6):513-22. PubMed PMID: 20006840; PubMed Central PMCID: PMC2905238.


5: Gastaminza P, Whitten-Bauer C, Chisari FV. Unbiased probing of the entire
hepatitis C virus life cycle identifies clinical compounds that target multiple
aspects of the infection. Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):291-6. Epub
2009 Dec 7. PubMed PMID: 19995961; PubMed Central PMCID: PMC2806752.


6: Dreux M, Chisari FV. Autophagy proteins promote hepatitis C virus replication.
Autophagy. 2009 Nov;5(8):1224-5. Epub 2009 Nov 1. PubMed PMID: 19844160.


7: Dreux M, Gastaminza P, Wieland SF, Chisari FV. The autophagy machinery is
required to initiate hepatitis C virus replication. Proc Natl Acad Sci U S A.
2009 Aug 18;106(33):14046-51. Epub 2009 Aug 3. PubMed PMID: 19666601; PubMed
Central PMCID: PMC2729017.


8: Bukh J, Thimme R, Meunier JC, Faulk K, Spangenberg HC, Chang KM, Satterfield
W, Chisari FV, Purcell RH. Previously infected chimpanzees are not consistently
protected against reinfection or persistent infection after reexposure to the
identical hepatitis C virus strain. J Virol. 2008 Aug;82(16):8183-95. Epub 2008
Jun 11. PubMed PMID: 18550671; PubMed Central PMCID: PMC2519567.


9: Bobardt MD, Cheng G, de Witte L, Selvarajah S, Chatterji U, Sanders-Beer BE,
Geijtenbeek TB, Chisari FV, Gallay PA. Hepatitis C virus NS5A anchor peptide
disrupts human immunodeficiency virus. Proc Natl Acad Sci U S A. 2008 Apr
8;105(14):5525-30. Epub 2008 Mar 31. PubMed PMID: 18378908; PubMed Central PMCID:
PMC2291127.


10: Cheng G, Montero A, Gastaminza P, Whitten-Bauer C, Wieland SF, Isogawa M,
Fredericksen B, Selvarajah S, Gallay PA, Ghadiri MR, Chisari FV. A virocidal
amphipathic {alpha}-helical peptide that inhibits hepatitis C virus infection in
vitro. Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3088-93. Epub 2008 Feb 19.
PubMed PMID: 18287023; PubMed Central PMCID: PMC2268589.


11: Gastaminza P, Cheng G, Wieland S, Zhong J, Liao W, Chisari FV. Cellular
determinants of hepatitis C virus assembly, maturation, degradation, and
secretion. J Virol. 2008 Mar;82(5):2120-9. Epub 2007 Dec 12. PubMed PMID:
18077707; PubMed Central PMCID: PMC2258938.


12: Law M, Maruyama T, Lewis J, Giang E, Tarr AW, Stamataki Z, Gastaminza P,
Chisari FV, Jones IM, Fox RI, Ball JK, McKeating JA, Kneteman NM, Burton DR.
Broadly neutralizing antibodies protect against hepatitis C virus quasispecies
challenge. Nat Med. 2008 Jan;14(1):25-7. Epub 2007 Dec 6. PubMed PMID: 18064037.


13: Guidotti LG, Chisari FV. Immunobiology and pathogenesis of viral hepatitis.
Annu Rev Pathol. 2006;1:23-61. Review. PubMed PMID: 18039107.


14: Pedersen IM, Cheng G, Wieland S, Volinia S, Croce CM, Chisari FV, David M.
Interferon modulation of cellular microRNAs as an antiviral mechanism. Nature.
2007 Oct 18;449(7164):919-22. PubMed PMID: 17943132; PubMed Central PMCID:
PMC2748825.


15: Sakai A, Takikawa S, Thimme R, Meunier JC, Spangenberg HC, Govindarajan S,
Farci P, Emerson SU, Chisari FV, Purcell RH, Bukh J. In vivo study of the HC-TN
strain of hepatitis C virus recovered from a patient with fulminant hepatitis:
RNA transcripts of a molecular clone (pHC-TN) are infectious in chimpanzees but
not in Huh7.5 cells. J Virol. 2007 Jul;81(13):7208-19. Epub 2007 Apr 4. PubMed
PMID: 17409145; PubMed Central PMCID: PMC1933310.


16: Uprichard SL, Chung J, Chisari FV, Wakita T. Replication of a hepatitis C
virus replicon clone in mouse cells. Virol J. 2006 Oct 28;3:89. PubMed PMID:
17069661; PubMed Central PMCID: PMC1635043.


17: Kapadia SB, Barth H, Baumert T, McKeating JA, Chisari FV. Initiation of
hepatitis C virus infection is dependent on cholesterol and cooperativity between
CD81 and scavenger receptor B type I. J Virol. 2007 Jan;81(1):374-83. Epub 2006
Oct 18. PubMed PMID: 17050612; PubMed Central PMCID: PMC1797271.


18: Sainz B Jr, Chisari FV. Production of infectious hepatitis C virus by
well-differentiated, growth-arrested human hepatoma-derived cells. J Virol. 2006
Oct;80(20):10253-7. PubMed PMID: 17005703; PubMed Central PMCID: PMC1617281.


19: Gastaminza P, Kapadia SB, Chisari FV. Differential biophysical properties of
infectious intracellular and secreted hepatitis C virus particles. J Virol. 2006
Nov;80(22):11074-81. Epub 2006 Sep 6. PubMed PMID: 16956946; PubMed Central
PMCID: PMC1642172.


20: Zhong J, Gastaminza P, Chung J, Stamataki Z, Isogawa M, Cheng G, McKeating
JA, Chisari FV. Persistent hepatitis C virus infection in vitro: coevolution of
virus and host. J Virol. 2006 Nov;80(22):11082-93. Epub 2006 Sep 6. PubMed PMID:
16956932; PubMed Central PMCID: PMC1642175.


21: Cheng G, Zhong J, Chisari FV. Inhibition of dsRNA-induced signaling in
hepatitis C virus-infected cells by NS3 protease-dependent and -independent
mechanisms. Proc Natl Acad Sci U S A. 2006 May 30;103(22):8499-504. Epub 2006 May
17. PubMed PMID: 16707574; PubMed Central PMCID: PMC1482521.


22: Zhong J, Gastaminza P, Cheng G, Kapadia S, Kato T, Burton DR, Wieland SF,
Uprichard SL, Wakita T, Chisari FV. Robust hepatitis C virus infection in vitro.
Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9294-9. Epub 2005 Jun 6. PubMed
PMID: 15939869; PubMed Central PMCID: PMC1166622.


23: Robek MD, Boyd BS, Chisari FV. Lambda interferon inhibits hepatitis B and C
virus replication. J Virol. 2005 Mar;79(6):3851-4. PubMed PMID: 15731279; PubMed
Central PMCID: PMC1075734.


24: Kapadia SB, Chisari FV. Hepatitis C virus RNA replication is regulated by
host geranylgeranylation and fatty acids. Proc Natl Acad Sci U S A. 2005 Feb
15;102(7):2561-6. Epub 2005 Feb 7. PubMed PMID: 15699349; PubMed Central PMCID:
PMC549027.


25: Kapadia SB, Brideau-Andersen A, Chisari FV. Interference of hepatitis C virus
RNA replication by short interfering RNAs. Proc Natl Acad Sci U S A. 2003 Feb
18;100(4):2014-8. Epub 2003 Feb 3. PubMed PMID: 12566571; PubMed Central PMCID:
PMC149950.


26: Thimme R, Bukh J, Spangenberg HC, Wieland S, Pemberton J, Steiger C,
Govindarajan S, Purcell RH, Chisari FV. Viral and immunological determinants of
hepatitis C virus clearance, persistence, and disease. Proc Natl Acad Sci U S A.
2002 Nov 26;99(24):15661-8. Epub 2002 Nov 19. PubMed PMID: 12441397; PubMed
Central PMCID: PMC137773.


27: Su AI, Pezacki JP, Wodicka L, Brideau AD, Supekova L, Thimme R, Wieland S,
Bukh J, Purcell RH, Schultz PG, Chisari FV. Genomic analysis of the host response
to hepatitis C virus infection. Proc Natl Acad Sci U S A. 2002 Nov
26;99(24):15669-74. Epub 2002 Nov 19. PubMed PMID: 12441396; PubMed Central
PMCID: PMC137774.