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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
# O& m; p/ }& U$ v  W/ t/ A0 FYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*

Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
key role in controlling viral infections. In this study, we report the cloning, expression, and
4 w! X5 X* }  A9 }+ a: vbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
9 n% k; q: H) U6 D6 T2 @5 G: }/ ZIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
4 l. {1 ~5 W; n  N3 \/ w6 [8 E4 Dactivity in microbats, which has important implications for virus interactions with these hosts.
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/ m/ {5 R* X& u4 \) wKeywords: bat; interferon; IFN-stimulated genes; antiviral activity


中文信息：

大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*


摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。


7 m( `! ^% }7 h8 b) a关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR

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