禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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2 ~; P8 s; Z( U3 b7 c' k9 ]08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
, O. @( i- l8 m8 t0 G【简    介】：
- Z4 J9 m, }( A/ [3 w9 V   08新书,大家共享    附件PDF下载！
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   The aim of this book is to create a resource that includes both basic methods that
( M; [- p) m1 N' D( |are currently used and well established as well as some of the most exciting new
1 R6 `8 y# p$ {1 F$ M/ p" o: kmethods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
0 r& O7 C3 V! b! K' xwill be of interest to both diagnosticians and researchers, but it does assume a basic
+ y7 V0 H  O! V' m5 X; ~knowledge of virology and molecular biology.
" w- y' ]9 L7 W0 EI would like to thank all the authors for their contributions. I am also thankful to
John M. Walker, the series editor, for his help in editing and working with me
through this project.
Erica Spackman
8 E& l' y2 y+ [) k. F0 b, `Athens, GA, September 2007

【出版日期】：2008
【目    录】：
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
" r. U2 M+ G: @; W  O% {: \1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
2 Q2 Y7 r. z% s* nErica Spackman
' @: Y9 V' `9 m4 h) B- q2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
  z9 w3 w% J% d9 j. Z1 Y7 _# H+ hMary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
& ]" d# d* [3 Z( w) a2 T" N4 uand Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
' G' |  ^6 X6 m( g/ S/ T8 i6 EErica Spackman and David L. Suarez
$ A  C/ X9 O2 E7 s. b4 Type A Infl uenza Virus Detection and Quantitation
by Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
& E+ _+ n: P) lSubtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
" Y0 h5 s* n9 w2 d6 w7 tErica Spackman and David L. Suarez
4 R9 j9 W6 f* y6 Avian Infl uenza Virus Isolation and Propagation
* c( g- a" [+ @" E! p1 kin Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Peter R. Woolcock
& D2 k+ S/ T- {1 Z) W- i& ?, k. S% ?! j7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
% \% o7 Y0 `' K" ^6 S# P8 @2 oMary Lea Killian
" g% f# i) @9 y8 X8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
/ a% u1 R5 V+ h) D1 k2 C- `. D# YSubtype Identifi cation and the Detection and Quantitation
4 `1 @& H9 N& _; Dof Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
Janice C. Pedersen
# `2 F6 H% p; g: X' Zvii
  B: u, t' c' D. I- j/ o2 i$ W9 Neuraminidase-Inhibition Assay for the Identifi cation
0 B* E& \3 K- K, ~3 `- rof Infl uenza A Virus Neuraminidase Subtype or
Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
' V  k! v( c. c2 P* t" EJanice C. Pedersen
9 V* ^" n3 `" D1 a! H: O7 e7 F10 Immunohistochemical Staining for the Detection of the
Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
( t. A# g+ e- j/ c. Y( D( ]Mary J. Pantin-Jackwood
11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
: h$ g% n, _1 l+ Q/ q; v, y' k; E" g# YJustin D. Brown and David E. Stallknecht
+ u1 G  y0 R6 H3 N( Q1 E12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
$ J" B' \- A% n' x0 NChang-Won Lee and David L. Suarez
& _5 t* R3 l& _) X4 a3 i13 Evaluating the Cell-Mediated Immune Response of Avian
Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
9 F) [! _- J6 F% L! r; tFollowing Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
9 `" q0 |* Q( x! XDarrell R. Kapczynski and Michael H. Kogut
- [$ g& y/ S" g3 o. D8 u) NIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents
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