禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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7 w0 _, c9 K5 Q: d( M( h08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
4 L* T9 Y; V! N. t' g$ ?5 \1 M/ P【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
! N* I4 |5 b: u0 y4 D6 s( R【简    介】：
: i3 Q- m5 f  f, H% I$ W   08新书,大家共享    附件PDF下载！
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   The aim of this book is to create a resource that includes both basic methods that
are currently used and well established as well as some of the most exciting new
7 J; p! x; [; Q  R3 e6 n' D6 h+ Hmethods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
: \) ^* {( @6 e7 ^* @will be of interest to both diagnosticians and researchers, but it does assume a basic
/ M. J* ^4 b9 \0 B3 `3 E% oknowledge of virology and molecular biology.
; M) ~7 ]" `4 k% M1 wI would like to thank all the authors for their contributions. I am also thankful to
( H0 k0 O$ V4 \/ `4 _$ SJohn M. Walker, the series editor, for his help in editing and working with me
through this project.
+ |% `" N3 X3 ?  m2 sErica Spackman
Athens, GA, September 2007

; v9 r- X$ V% Z9 i9 U5 q2 ~【出版日期】：2008
) R4 ^5 M" T9 y【目    录】：
6 G9 l, z* o0 _* iPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
/ D2 s1 ^$ I5 `& Y; V9 oErica Spackman
2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
  B6 E" Z, `' T2 P& T; DMary Lea Killian
& Z/ B! G9 g/ s3 Avian Infl uenza Virus RNA Extraction from Tissue
8 d2 X# O/ t1 k' i% Z( Q' Kand Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
& B7 |( R, f. O- R0 j$ T- o9 t4 Type A Infl uenza Virus Detection and Quantitation
; J# ^& t/ D2 l$ w! r6 S1 wby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
4 O# a" r0 p. M/ U5 e+ Y5 Detection and Identifi cation of the H5 Hemagglutinin
Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
! l" q( u; o" c4 ^: j6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Peter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
Mary Lea Killian
" n7 u2 s% s1 e, |# e4 P8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
Subtype Identifi cation and the Detection and Quantitation
of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
8 @' T: k9 F% T4 a5 b9 s( MJanice C. Pedersen
1 q  n# p6 Q; R" D9 ?) Bvii
+ n0 o4 y6 b/ b# G9 Neuraminidase-Inhibition Assay for the Identifi cation
of Infl uenza A Virus Neuraminidase Subtype or
Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
+ R/ q2 G$ Q( `" e% fJanice C. Pedersen
% Z: W6 D2 v; M; u10 Immunohistochemical Staining for the Detection of the
9 J1 y% Y/ x: ]% z% K: ZAvian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
1 X, p/ T: N6 k5 {0 `# ^+ ZMary J. Pantin-Jackwood
  R/ @$ ~" q3 t6 N( m# A11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
4 ~: W& V7 A+ U  l1 KJustin D. Brown and David E. Stallknecht
6 t: _" B. H) \4 ?. \12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
9 {# J' p5 \+ ]: `! F3 C  M4 VChang-Won Lee and David L. Suarez
13 Evaluating the Cell-Mediated Immune Response of Avian
; |$ P/ n6 v3 H$ _$ o$ gSpecies to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
$ ?6 q1 {# M+ Z$ e3 N3 I- R& mDarrell R. Kapczynski and Michael H. Kogut
) c7 u, l- h2 ~% CIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents




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