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IMMUNOLOGY
- N( C& C" L0 d% O F* @2 }60.401
! l2 X" F+ t- DLAB MANUAL 2004
0 C' w( I3 {& S1 ?不错的书,下面是目录 ( t% U, P9 U6 K: A6 C# x4 m
SCHEDULE 2
) S: S0 `% b( F& PGENERAL INSTRUCTIONS 4
* ~* g6 r! g4 B7 b" K2 vREFERENCE BINDER CONTENTS 8: a5 W4 ]/ D3 T$ ~1 m9 R" p4 N
STANDARD OPERATIONS PROCEDURE 9
3 A7 t% m7 }+ G+ o# u( H1 d7 DWHMIS 117 l& W& m' o# i4 m1 n9 r( ^
Lab 1: Preparation of Crude IgG by Salt Fractionation 13; _9 O! t0 w/ s1 T& h: w7 M6 J h
Lab 2: Immunoprecipitation 17! `1 Q3 u4 X* R- S
Lab 3: Hemagglutination Assay 284 i. c o% {. ?: a
Lab 4: Introduction to the Enzyme Linked Immunosorbent Assay (ELISA) 33
3 `4 N4 x$ X7 L% H+ cLab 5: Part I: Induction and characterization of a primary cytokine 38
# }; i" [+ }+ f* Bresponse in vivo.4 J' q7 P0 `1 i* f( N
Part II: ELISA precision training.; C [0 U) g, m+ N
Lab 5: Part III: ELISA ASSAY- Quantitative analysis of IL-13 Response 47; r5 r3 \7 e+ B) z
Appendix:7 r, Z4 d0 W8 Z' D' Z! x
Solutions 53
2 n! o$ D) Y$ @/ WFloor Model Centrifugation Operation 545 V6 L% P) f% T, o- K; ^& P
Pipetman Operation 56( T+ A, w3 h! `
Electrophoresis 58
& M) o! U* r( Q& b% u" p0 lSample Lab Exam 60 |
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