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发表于 2015-2-3 19:20:34
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7 P- U! H, ]1 M. C8 T3 B B) a+ b3 |% l8 ?" _: c, T, ` D* B
IMMUNOLOGY
* c# E0 A: b. O* y4 I60.401
" q9 w* X' X- z3 ~! o8 z" RLAB MANUAL 2004
) \1 H; S2 H; I不错的书，下面是目录
9 I4 A% L6 Y: n* U7 BSCHEDULE 2
& X; @. Z' p) v6 yGENERAL INSTRUCTIONS 4 ( l6 ~ F3 O$ \* L: s
REFERENCE BINDER CONTENTS 8
) d" C T0 `6 D! `5 C* _STANDARD OPERATIONS PROCEDURE 9
9 D+ X# H+ ?" S7 g. q* ?WHMIS 11; A4 _# l3 f$ X" f' V$ A. ?
Lab 1: Preparation of Crude IgG by Salt Fractionation 13( M3 ` S! j9 p s- ~3 M8 |
Lab 2: Immunoprecipitation 17. n( _7 E8 p( M0 l0 ?" _
Lab 3: Hemagglutination Assay 28
% a( o: o% [9 V( z8 W/ }1 B1 l7 QLab 4: Introduction to the Enzyme Linked Immunosorbent Assay (ELISA) 33
. O, [8 `3 ?9 `, |. k4 qLab 5: Part I: Induction and characterization of a primary cytokine 38
. N0 `, A0 k+ \, V; F' Lresponse in vivo.
3 q3 i- t) a0 tPart II: ELISA precision training.5 B: g+ t9 I) @" _
Lab 5: Part III: ELISA ASSAY- Quantitative analysis of IL-13 Response 47& r H2 l" z. s; r
Appendix:
3 ^% ~ [7 r D. s# j% G$ y( uSolutions 53
% ]4 {7 v- E" C KFloor Model Centrifugation Operation 54
9 O" O+ `5 [) `& DPipetman Operation 56
) v5 Z5 H) m7 V v! P# l, ~2 ~Electrophoresis 58, M9 s) I& r0 m" T3 P7 ?
Sample Lab Exam 60 |
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发表于 2015-2-3 19:18:57
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