Extracting Viral RNAs from Plant Protoplasts

jbclinnic

电子书：
The analysis of viral RNA is a fundamental aspect of plant RNA virus research. Studies
that focus on viral RNAs often involve virus infections of plant protoplasts .
Protoplast offer the advantage of simultaneous initiation of infections,
which allows for superior temporal and quantitative analyses of viral RNAs. The efficient
isolation of intact viral RNA is key to any such investigations. This unit describes
two basic protocols for extracting viral RNAs from plant protoplasts. An approach for
preparing double-stranded viral RNA from total RNA pools is also provided. The viral
RNA prepared by using these techniques can be used for further analyses such as primer
extension, reverse transcription–PCR, and northern blotting.