科学家称新检测法可排查所有病毒

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医生要治疗病人，必须先找出确切病因。如果是病毒作怪，现有的诊断性化验往往不够灵敏，从而无法检测出致病病毒。或者医生必须首先怀疑到是哪种病毒引起症状，然后才能开具化验单予以确认。然而，华盛顿大学圣路易斯分校的研究人员开发出一种新的检测方法，或许解决了这个难题。

据美国《大众科学》月刊网站10月2日报道，ViroCap是一种新的诊断性检测，可验明感染人和动物的任何病毒。他们的研究结果发表在《基因组研究》杂志上。

研究报告的第一作者格雷戈里·斯托奇说：“有了这个检测法，你不必知道自己要找什么。”

报道称，为开发这个检测法，研究人员收集了能感染人和动物的每种已知病毒与众不同的DNA和RNA序列——迄今为止纳入了多达200万件独特的遗传物质。检测时，需要把这些独特的序列与患者血液、粪便或唾沫里的遗传物质进行比对。如果有匹配，就用高通量基因测序技术分析该物质，从而做出诊断。

研究人员希望利用这一新技术来检测致命病毒如埃博拉病毒、马尔堡病毒和SARS以及能引起严重胃肠道感染的病毒。

来源：参考消息网

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Enhanced virome sequencing through solution-based capture enrichment

Todd N Wylie1, Kristine M Wylie, Brandi N Herter and Gregory A Storch

Metagenomic shotgun sequencing (MSS) is an important tool for characterizing viral populations. It is culture-independent, requires no a priori knowledge of the viruses in the sample, and may provide useful genomic information. However, MSS can lack sensitivity and may yield insufficient data for detailed analysis. We have created a targeted sequence capture panel, ViroCap, designed to enrich nucleic acid from DNA and RNA viruses from 34 families that infect vertebrate hosts. A computational approach condensed nearly 1 billion base pair (bp) of viral reference sequence into less than 200 million bp of unique, representative sequence suitable for targeted sequence capture. We compared the effectiveness of detecting viruses in standard MSS versus MSS following targeted sequence capture. First, we analyzed two sets of samples, one derived from samples submitted to a diagnostic virology laboratory and one derived from samples collected in a study of fever in children. We detected 14 and 18 viruses in the two sets, comprising 19 genera from 10 families, with dramatic enhancement of genome representation following capture enrichment. The median fold-increases in percent viral reads post-capture were 674 and 296. Median breadth of coverage increased from 2.1% to 83.2% post-capture in the first set and from 2.0% to 75.6% in the second set. Next, we analyzed samples containing a set of diverse anellovirus sequences and demonstrated that ViroCap could be used to detect viral sequences with up to 58% variation from the references used to select capture probes. ViroCap substantially enhances MSS for a comprehensive set of viruses and has utility for research and clinical applications.

http://genome.cshlp.org/content/ ... 9.115.full.pdf+html

ghx0123

希望这种检测方法能成为现实

hantavirus

基于大规模测序技术？好普及吗？

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hantavirus 发表于 2015-10-8 22:28
基于大规模测序技术？好普及吗？

恩，类似体外诊断，在医院里可以普及啊，做个血检等就可以了