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【书名版次】: Adeno-Associated Virus: Methods and Protocols (Methods in Molecular Biology) 9 V1 W: l1 @* t. c: X
【作 者】:Richard O. Snyder (Editor), Philippe Moullier (Editor)
$ P! B! d) }2 E" \【出 版 社】: Humana Press; 2011 edition
6 T6 g* ~: y9 m3 p. C【出版日期】: (November 30, 2011) ' g2 p% Q9 O) p8 D+ i' N p' _
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【简 介】: Today, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding — or at least an appreciation of — the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Protocols, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Adeno-Associated Virus: Methods and Protocols provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.& W1 l. R$ l0 m) j( _
【目 录】:
# N K# Q" }5 P1 b# U5 DHardcover: 475 pages, `8 |6 q, J1 \0 t# I5 d
Language: English
* Q* h3 h" f) E: O c3 _ISBN-10: 1617793698
# t- s$ Y; p7 P) {1 z' [ISBN-13: 978-1617793691
( {. k. c* u$ B/ X# @下载地址:# C1 c0 E1 | S+ G( {
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