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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)
2 H. g: j" X7 s& \; UYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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4 L8 `$ U" j( v" LAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
6 j1 P* x# b8 u% xTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
1 d( _- J* t, k; pinformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
m, q2 e/ w2 G, z# X/ ukey role in controlling viral infections. In this study, we report the cloning, expression, and7 _$ E& g3 h9 V1 r
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We3 x5 n4 A) t& N( M1 c6 R/ F* a
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
5 O6 o/ {7 K8 a5 d- _9 U0 P' X9 D% aM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
& r2 c' ?1 ?; C/ g' o' c5 c* }IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat+ g) u6 S9 o0 G/ O
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
, Y$ K& H% l! cactivity in microbats, which has important implications for virus interactions with these hosts.
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Keywords: bat; interferon; IFN-stimulated genes; antiviral activity2 N* U4 p, a; J- y: Q1 h
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中文信息：; @5 o% t# |$ ~# ]
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大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*5 f' j% P5 V3 m9 M% R4 ^( z
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/ e/ ~$ p; p4 }9 D$ r6 I摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。9 g8 J5 v1 X* }/ A( m
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关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR, T1 v, V- K2 c( x& X4 l. V
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
