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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)
8 ~1 T \ {6 M2 n' l4 ?Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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" n4 [" |1 q7 U$ m: ?8 d% aAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.4 G: R2 Z$ f6 {1 y
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little+ l. [4 k, ?3 m* e
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a) x+ T: x$ `7 I( d9 I7 Q
key role in controlling viral infections. In this study, we report the cloning, expression, and) g" u8 N/ E" d& H+ p& x
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
|) r/ B. |% Ddemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from5 K1 H- Y* f1 x1 ^7 R3 ^8 w
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant1 l3 V: F9 S5 e$ d
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
: }: W9 j8 _$ h* e; e! hspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral B' q G) W8 E# z
activity in microbats, which has important implications for virus interactions with these hosts.0 | E2 g( L# d; y
" S* ~0 N1 v9 N0 z/ k( }) K0 eKeywords: bat; interferon; IFN-stimulated genes; antiviral activity5 O9 K4 G- a8 B% _: }: \
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中文信息：/ _9 ]! ^& y9 Q S- a5 c
1 c. J' `5 f$ t4 B大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测8 \! f+ a0 l( j! C. g% \. ?
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梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
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7 H, M1 Y+ K; F摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。- w& B. w ]- N9 x+ B9 b
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: L# m+ T; P6 ^6 e3 M关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
