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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)
, [# V# \) v ^Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*% Z# D) N- H" {0 a6 g. V* {4 l
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Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
2 j5 K* B: A8 d% S' OTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
: ?* R8 ~$ a' \3 Z8 ninformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a8 v) q E- @2 t/ ^; |$ E
key role in controlling viral infections. In this study, we report the cloning, expression, and) S" F; l& O, F$ r& U" @" ]
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
! H, o6 `& l6 E% y/ F. ~2 ?! idemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
" _) O% f2 `7 C- Q j* H9 z2 Z! qM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
% I8 J# q' B1 C h6 vIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
m9 Q: d3 o1 J1 I+ d6 Ispecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
( w* `% Q! K( factivity in microbats, which has important implications for virus interactions with these hosts.0 u' _3 m9 D( _4 H$ t
) g# S% Q5 _ p1 J! L* ~2 K& ~* |Keywords: bat; interferon; IFN-stimulated genes; antiviral activity
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中文信息：
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& d* W* C$ G: h& A大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测- }: J# E. [5 V! p7 e$ `- }5 x, m
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2 v9 C5 M }; X; h' G% n! i7 }梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*% L4 t7 h. G+ V4 V3 e
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& g2 L; c7 ]* x' l6 X* a摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。+ e6 ^% T$ {. j
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1 U, B. W4 h- V( ^- ?关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR. m3 @4 p5 H2 Q8 |7 H% H' B% d; S
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
