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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)/ }+ c4 Z+ N9 P
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.# ^( V7 j4 c: a
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little
0 Q& x/ t" ^ z/ K; ]/ `information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a! P; x( ~0 C& }3 \9 T" l5 J- }2 ?
key role in controlling viral infections. In this study, we report the cloning, expression, and
( o/ @/ I" r3 abiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
6 q1 Z1 R# j: a; a) X: Ademonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
# j- F9 H* `3 C( a9 R) z7 F$ }M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant8 }4 |3 f/ b% [- P3 z3 I! o
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat* a/ x* X. \3 k
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral. S4 f7 s( }& Z0 ^
activity in microbats, which has important implications for virus interactions with these hosts.
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: ~, q* R, D/ t6 h' W* }$ p4 J' gKeywords: bat; interferon; IFN-stimulated genes; antiviral activity
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7 u" b2 w3 \1 Z8 r \8 v中文信息：, E, N y$ p9 ?0 r% X5 h# w% q# B
6 S. ?- u S0 { e2 x# ?0 Z大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*" y5 I9 }' d, I- p: F4 q
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. R( _9 o5 \0 @. h: i/ t7 ]% i, d摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
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) k7 ]& r4 N( ^3 f$ c* O2 a关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR* f; U/ a! U, i" ]3 e7 b
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
