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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*

Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little
3 h6 J# ?; p' K. v8 Binformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
key role in controlling viral infections. In this study, we report the cloning, expression, and
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
! D. T& ~- p2 Q( t- L+ `demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
3 z% X$ y& \0 S$ I3 I+ U; \& P, fM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
5 h3 a7 s# x9 ^7 c1 e# \2 nIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
8 h! ?' V: P/ M# jspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
' t1 d' _( ^+ w9 Yactivity in microbats, which has important implications for virus interactions with these hosts.
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! ^  C/ Y! o2 ?" |2 h3 z+ }Keywords: bat; interferon; IFN-stimulated genes; antiviral activity


中文信息：
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大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测

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梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*

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: m- G* N9 u. H( a- z摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。


& ~" _, y0 Q- `! \5 V关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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