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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)2 @$ e" N3 l3 H) A# `/ w9 D$ a7 x
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
5 C5 K: N8 a2 RTherefore, bats may have evolved effective mechanisms to control viral replication. However, little* e+ x/ Y1 }0 b$ }0 w8 q" O. s1 N
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
0 p [" ~! Y3 f P$ p% g; d' Xkey role in controlling viral infections. In this study, we report the cloning, expression, and
$ X. j; z4 E. A- v$ x$ j. Pbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We: e q. D) H; w; g
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from+ ?$ q g6 X, }+ p. }' n$ Y4 G3 E2 G2 f
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
6 S, _7 q) H* T( B" u7 {IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
# J! L9 L- _3 p/ k5 J; kspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
J4 f! m! Z0 {$ L- P* {- E# j8 xactivity in microbats, which has important implications for virus interactions with these hosts.( T# P; n6 d1 v- H! t9 u
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Keywords: bat; interferon; IFN-stimulated genes; antiviral activity$ H2 W$ H/ z0 A, A8 F( v, L
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& b& A' G$ p4 E$ z; e2 q5 Q! b中文信息：8 s3 Z1 x! V' v! C" Y
5 _, T5 K- w3 m m j) v) \大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测 u' B9 t& r0 z8 Q) R2 _" L! {
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# s2 y5 ?8 S* [0 S& Y梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
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摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
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) s# Y* ]2 [" ~% d6 R关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
