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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)8 U8 ^1 S& c/ P' J' c) z9 H
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*7 C9 }8 Y3 Z- M9 |
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Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
6 L& Y/ I# f2 {. {9 WTherefore, bats may have evolved effective mechanisms to control viral replication. However, little: e5 e9 ]; Z; s! w" Y6 d
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
1 o2 _% N$ s: f% _key role in controlling viral infections. In this study, we report the cloning, expression, and
, ^2 p5 ]# c& d! U7 bbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
Z4 I) Y. J- }' W- R2 V. Xdemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from3 ^' L( _/ a6 z+ t/ C* }
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant1 i4 ?8 ~3 `2 w9 M. z
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
! L5 T5 p$ j+ vspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
1 T& U, \# B& oactivity in microbats, which has important implications for virus interactions with these hosts.
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) r; ?# G9 {/ D$ _: B3 \Keywords: bat; interferon; IFN-stimulated genes; antiviral activity
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中文信息：
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% i( g/ m( o) [ e; \6 M% y1 ?! e g大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测2 C# V2 K a* |' R
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9 ^: c. [. v. ?! }- o/ c梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
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4 Q* M& B( J' c$ f7 Y7 x6 I. n摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。& d# S/ ?7 c" Q+ W. l7 J* s
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关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
