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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)
9 I! M9 c. K' F5 A8 qYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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; R# `; G: r; ]) b- hAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
2 ?# O9 c: D* z" O( Y% X9 JTherefore, bats may have evolved effective mechanisms to control viral replication. However, little9 U0 Q; A$ q7 H# i
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a, |5 R I& E) u# { k1 d/ @! R3 ?3 A
key role in controlling viral infections. In this study, we report the cloning, expression, and
7 s; f2 \5 ^# n" ?0 ?& W K3 h! w) zbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We0 K" s! Y' H4 G( @ T3 }
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from% ?" v( Q( D& z( n0 ]: y
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
0 F' J: e: z! P) k- YIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat l4 i5 F4 _( F7 o! U! E* ]
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
- U: V3 y! X3 s, Lactivity in microbats, which has important implications for virus interactions with these hosts.
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6 \; Z6 D3 W4 |7 }Keywords: bat; interferon; IFN-stimulated genes; antiviral activity7 A4 ?" A/ c7 l1 b q1 }. U
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' s+ g' D5 p' Q. I8 S中文信息：
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' G7 n: w8 k: m3 C4 O9 g# U% s大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*% D7 J. ~& O5 b
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$ R8 Y" ?* p2 W% x摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。 S8 Y; Y6 f9 s; W |
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关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR! e/ S; C4 a% h2 }
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
