禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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; v  B7 Q8 B6 u! c" G* S08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
- l, |$ q& B5 G【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
【简    介】：
   08新书,大家共享    附件PDF下载！
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   The aim of this book is to create a resource that includes both basic methods that
- b& m, T* w& U0 ~are currently used and well established as well as some of the most exciting new
methods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
will be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
I would like to thank all the authors for their contributions. I am also thankful to
& y2 {% K/ F8 a8 Q7 Z+ `John M. Walker, the series editor, for his help in editing and working with me
3 M( J0 g. l+ m" _' jthrough this project.
9 Y7 R: V, V- yErica Spackman
Athens, GA, September 2007

5 R2 l) y4 Y/ M【出版日期】：2008
  h/ u! c- e) t* k' X【目    录】：
; F0 `! T0 N( FPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
6 H+ h  a* v. t: C* H1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
/ z- Z" d$ ]4 z" NErica Spackman
  N# I4 y% v% ?% D! j0 W" F0 d* V2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
1 z( Y7 G, B/ w9 \' Y2 {* I# EMary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
by Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
  L8 w* u' `' C6 ]5 Detection and Identifi cation of the H5 Hemagglutinin
- o: M2 S# I- `2 _/ t/ N7 Q" V; pSubtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
) q" S% I  R( Q0 P! ~& k+ f7 I" rErica Spackman and David L. Suarez
6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
7 I+ B* |3 N5 i5 u! q* cPeter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
  }  w* @/ l" \. h7 F+ q' `8 vMary Lea Killian
4 E5 W0 H& K: [& d8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
3 W3 [: b5 O, ^7 h$ gSubtype Identifi cation and the Detection and Quantitation
1 C4 q# g" {3 R% R% B% p( Tof Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
' Y( E  p- g7 J8 O9 g- \& L, yJanice C. Pedersen
/ t9 U- O2 P1 h+ n3 rvii
) O) a2 F. M* N8 p( Z% G+ o3 U9 Neuraminidase-Inhibition Assay for the Identifi cation
of Infl uenza A Virus Neuraminidase Subtype or
* g& \( o! T2 j& Y/ n6 ^0 hNeuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
, m% j0 q8 k8 J# \/ @Janice C. Pedersen
+ z4 K' M3 I: @, f8 q1 {' u: P% E* t10 Immunohistochemical Staining for the Detection of the
1 |4 ~8 I* ~8 bAvian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
3 w7 _9 n* y% z' ?3 {: {Mary J. Pantin-Jackwood
0 b% Y# _1 o4 J9 b5 r11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
  Z" }- |* Z' e! x4 `! Q9 }) rJustin D. Brown and David E. Stallknecht
. v+ I6 @. \  X1 l2 U7 ~/ Z  @3 }12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
13 Evaluating the Cell-Mediated Immune Response of Avian
Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
$ C* O4 J2 s; [% W% y& ?) Z  YDarrell R. Kapczynski
& \; z; ]0 L  M9 o$ A14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
/ q2 Y. {5 D% B5 P' C2 nDarrell R. Kapczynski and Michael H. Kogut
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents
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