禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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08年新书 Avian Influenza Virus
8 y$ a- B0 I1 ?: k1 A2 P4 {3 o【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
1 `. T' W* O0 `. N【作    者】：Erica Spackman Editor
0 ]7 r) q/ W, W  ?& }【出 版 社】: Humana Press
【简    介】：
   08新书,大家共享    附件PDF下载！

, Z+ B5 Q+ o6 Y; M0 m   The aim of this book is to create a resource that includes both basic methods that
are currently used and well established as well as some of the most exciting new
4 f0 T' {% ]- g9 }5 Z% V5 J4 _& Gmethods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
5 [/ ?: N: M& w) |- E& b" iwill be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
5 Y. V/ f; [8 j) ~3 S8 y* [I would like to thank all the authors for their contributions. I am also thankful to
; x2 c0 O' E* [" aJohn M. Walker, the series editor, for his help in editing and working with me
' b. L4 m4 `* d3 x1 W1 n  Rthrough this project.
Erica Spackman
# N- N( p7 C, j+ \9 iAthens, GA, September 2007

【出版日期】：2008
【目    录】：
! U  _! L0 R' e  p- PPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
2 o# C2 M' V, DContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
: F6 X% l. j% y) JErica Spackman
2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
/ O; D) W9 ]4 `7 A  Z7 ]2 J& jMary Lea Killian
8 o9 M- w' Q6 `: K1 i3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
0 |* J% z6 s/ T" a- j; vby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
& C5 j5 |& x3 |* e+ \Erica Spackman and David L. Suarez
3 E& a! O% U  o+ u9 h5 Detection and Identifi cation of the H5 Hemagglutinin
4 d' z7 {; }4 h/ e- MSubtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
+ R7 R. j0 w( R0 d6 ZErica Spackman and David L. Suarez
% ^/ Z, r/ }8 P. l6 Avian Infl uenza Virus Isolation and Propagation
& e2 ^+ P3 m) \/ J, }- ~- kin Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Peter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
Mary Lea Killian
6 U* ?$ e' S+ r7 B! c8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
Subtype Identifi cation and the Detection and Quantitation
of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
& I) Y! s! P0 o* F& GJanice C. Pedersen
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9 Neuraminidase-Inhibition Assay for the Identifi cation
- I, T1 C0 E, p( s$ o( k  aof Infl uenza A Virus Neuraminidase Subtype or
Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
" N9 p  o# ~. R3 i/ F4 OJanice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
& h% I; R. j+ ~) u4 ~Mary J. Pantin-Jackwood
7 H& C9 m3 V7 X11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
2 X' E; {4 Z- L12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
13 Evaluating the Cell-Mediated Immune Response of Avian
( M, ?4 C4 [. i: kSpecies to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
0 H9 C, A$ I0 G7 gFollowing Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
. {% S, Z+ [5 o5 zIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
3 G3 _( R  ]6 X+ Lviii Contents
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