禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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08年新书 Avian Influenza Virus
. e4 z3 U& V0 D/ K$ Z% k【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
【简    介】：
% T5 @% C$ ]# P( K0 F! ^   08新书,大家共享    附件PDF下载！

; j( [) y5 J9 x/ u( u- \" t   The aim of this book is to create a resource that includes both basic methods that
0 F5 t7 b) ?1 F& |are currently used and well established as well as some of the most exciting new
methods for studying the virus itself, and to include methods that focus on work
4 ?% @  x( s1 y2 O! ~with avian hosts, an area that has been greatly lacking. In that context, this volume
* Y* D4 ~, M0 g$ w. B, R2 lwill be of interest to both diagnosticians and researchers, but it does assume a basic
  I% K, U" m- v4 R0 m0 Kknowledge of virology and molecular biology.
I would like to thank all the authors for their contributions. I am also thankful to
John M. Walker, the series editor, for his help in editing and working with me
1 j& D" p7 R# w  S0 z2 p8 Pthrough this project.
Erica Spackman
Athens, GA, September 2007

【出版日期】：2008
5 N9 U% ~. e4 X( J1 ^' S【目    录】：
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
. o6 L& \0 r" ^; h& ]3 WErica Spackman
: {# G, S3 N  H8 ~2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
! }6 ^1 L7 R8 eMary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
# w2 d. `; g' y0 q# m' E$ xand Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
+ Q* w) ^, p4 h8 m+ z0 |" v8 A4 Type A Infl uenza Virus Detection and Quantitation
by Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
- c6 M) T. ]- _5 |- h. mErica Spackman and David L. Suarez
+ q9 x! n% n3 y7 `1 q5 Detection and Identifi cation of the H5 Hemagglutinin
Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
' H6 z" q5 ^: z6 D: P- _6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
  I6 A" M% t9 a4 u5 T0 G/ G; |Peter R. Woolcock
9 ]. i) {8 z0 w  R7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
3 x. D$ f8 K0 XMary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
# ]8 e7 ?  P' F+ ]# @Subtype Identifi cation and the Detection and Quantitation
6 ^+ h' U. D, U+ H" D7 Uof Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
Janice C. Pedersen
vii
9 Neuraminidase-Inhibition Assay for the Identifi cation
: {. Q- B& }* g1 x$ c' n2 N' D' V' oof Infl uenza A Virus Neuraminidase Subtype or
$ \) Q: f; [/ Z: f+ t5 \) r4 ~Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
, w* Y' d: D* d2 L1 t4 V' ]7 B6 g' wJanice C. Pedersen
9 D1 L+ \! x% [  f( [10 Immunohistochemical Staining for the Detection of the
Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
* y3 @7 G9 L5 H/ }Mary J. Pantin-Jackwood
11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
/ d' j0 I9 k6 o! q  |Justin D. Brown and David E. Stallknecht
5 Z. P  j) c& O0 T+ o7 g4 _12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
! a7 V  a0 c+ D/ M2 U* m: LChang-Won Lee and David L. Suarez
6 s( X8 `8 X1 D7 C* a) B9 L13 Evaluating the Cell-Mediated Immune Response of Avian
3 x. T) l, Q& G, {: NSpecies to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
! B0 P7 q+ ]5 {Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
  J) e: I# P! a) v& Z4 [+ RIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents
2 P/ x  H" j, u2 s
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