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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
, H6 j: w( x$ S- C/ Q3 D! k) r( dTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
2 o* f1 x+ V( c. cinformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
key role in controlling viral infections. In this study, we report the cloning, expression, and
" n- L: F8 C( c. K: obiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
# L& K( f- M% |* T# `' ~6 BIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
# ?3 a$ k) i1 N6 Z9 m7 Xspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
) d2 ~& A! N% @7 S/ Bactivity in microbats, which has important implications for virus interactions with these hosts.
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  G% ~6 f0 V: H1 a  g# j* XKeywords: bat; interferon; IFN-stimulated genes; antiviral activity

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8 y" h1 e. u. K. B3 g- ~! D: V# z中文信息：
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* Y: K/ _* R; }. U2 M( K7 R, m- J大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测

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梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
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1 j9 P! B6 u5 @) A: {9 Y( G摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。


5 K3 ]7 Z$ B, B6 Z  [关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR

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