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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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+ X, }1 D* T4 ?( }  v0 B. JAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
! _) c1 l( z5 s$ g* H2 W6 VTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
1 \. }% X+ A" T/ v; _information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
6 U$ N) @0 g6 w, lkey role in controlling viral infections. In this study, we report the cloning, expression, and
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
8 q, z9 X. m) bM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
- q9 Z% w1 T) D1 s+ t0 t) c/ EIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
! q8 ^$ c9 f3 `- o* f) k( fspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
6 V( J& L! X% E. Uactivity in microbats, which has important implications for virus interactions with these hosts.
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Keywords: bat; interferon; IFN-stimulated genes; antiviral activity
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2 \5 m1 K! d& G; c中文信息：
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; f4 y4 x) Z. |6 e* G; o, E大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测


" `. _% s- Q3 ~& n$ P梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
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摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。


- Y) T* o, G6 S7 @关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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