|
|
Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF): Y; r+ A+ Y* Q- i8 ]
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
0 Q- F/ a% |. |* K5 k0 V" A; @3 H( c' s3 j+ B
Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.& S2 O" W8 ]# y/ x- ]# ?: t& Z5 [
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little: j+ D x( W3 g% L) k
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a4 q, G! [. S2 L: Z* r1 E# [2 o
key role in controlling viral infections. In this study, we report the cloning, expression, and
+ K; C* ?0 l R6 @# I! ?9 @& Xbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We5 A0 W8 E4 o/ s: R
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from( ?6 [( T# u4 e2 R" j
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant$ [1 }' }* H! ~/ ?: G
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat, q! H1 q, l: E8 A, [8 e& }, _
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral P+ T! z. l; F* g( C1 Y/ Y
activity in microbats, which has important implications for virus interactions with these hosts.2 M% z# U' G) t+ O
8 w2 D4 E& N: |% W* f2 XKeywords: bat; interferon; IFN-stimulated genes; antiviral activity
& x0 Y T! M7 n) ]# q0 K* a, p: \5 c' v
, B6 {' G3 O R" F% a% p& E中文信息：4 |; H4 R4 t1 \, H4 i
/ Z+ ?9 i0 A8 k' c# n- Z% \
大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测4 j( |. l( C% X5 ^. v$ }# k
' C; t) k: i" V/ r% o" | L9 h$ G9 W3 Z" ]: g2 v
梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
5 e2 r9 D; |1 h, F* u
* E# U3 s. I8 X& v' ?# ` U& V
. O6 B: o+ H M3 L摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
; K0 G) ]4 r s* m, |4 n# z3 @
6 }, ~ B! f1 ^# m2 b4 H2 @+ [! L K* e( U8 p
关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
& Z3 |& C+ G9 [( A0 f' f5 a
9 J) I. v, `. K2 S- F |
|
发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
